Rempuia Vanlal, Gurusubramanian Guruswami, Roy Vikas Kumar
Department of Zoology, Mizoram University, Aizawl, Mizoram 796004, India.
Department of Zoology, Mizoram University, Aizawl, Mizoram 796004, India.
Reprod Biol. 2024 Dec;24(4):100956. doi: 10.1016/j.repbio.2024.100956. Epub 2024 Sep 28.
Visfatin is expressed in the testis of chicken, humans and rodents; however, direct role of visfatin in the adult testis has not been studied. We investigated testicular responses after intra-testicular injection of FK866. The effects of visfatin inhibition were accessed at 24 hrs and 1 week post FK866 treatment. The testicular histoarchitecture were degenerated after 24 hrs of FK866 treatment along with supressed testosterone and proliferating markers and resumption in these parameters showed after 1 week. The expression of AR and ERα were down-regulated after 1 week of FK866 treatment. The expression of BCl2 was down-regulated along with a slight elevation of caspase3 after 24 hrs; however, both proteins still showed suppressed expression after 1 week. Furthermore, ERβ expression, 3βHSD, and 17βHSD were down-regulated in both groups compared to the control. Despite the down-regulation of some factors, the testicular proliferation and histoarchitecture showed resumption in the testis after 1 week of FK866 treatment. This could be due to increased testosterone secretion by suppressing aromatase expression. In conclusion, our result is the first report on the direct role of visfatin in the adult testis. Visfatin has a stimulatory role in testosterone synthesis and proliferation in the testis. Moreover, some deregulated factors in the testis after 1 week of FK866 treatment, despite normal histoarchitecture treatment, could be a compensatory mechanism after visfatin inhibitions.
内脂素在鸡、人类和啮齿动物的睾丸中表达；然而，内脂素在成年睾丸中的直接作用尚未得到研究。我们研究了睾丸内注射FK866后的睾丸反应。在FK866治疗后24小时和1周时评估内脂素抑制的效果。FK866治疗24小时后，睾丸组织架构退化，同时睾酮和增殖标志物受到抑制，1周后这些参数恢复。FK866治疗1周后，雄激素受体（AR）和雌激素受体α（ERα）的表达下调。FK866治疗24小时后，Bcl2的表达下调，同时半胱天冬酶3（caspase3）略有升高；然而，1周后这两种蛋白的表达仍受到抑制。此外，与对照组相比，两组中雌激素受体β（ERβ）、3β-羟基类固醇脱氢酶（3βHSD）和17β-羟基类固醇脱氢酶（17βHSD）的表达均下调。尽管一些因子下调，但FK866治疗1周后睾丸中的增殖和组织架构仍恢复。这可能是由于抑制芳香化酶表达导致睾酮分泌增加。总之，我们的结果是关于内脂素在成年睾丸中直接作用的首次报道。内脂素在睾丸睾酮合成和增殖中具有刺激作用。此外，FK866治疗1周后睾丸中一些失调的因子，尽管组织架构正常，可能是内脂素抑制后的一种代偿机制。
