宿主 miRNA 和 mRNA 谱在 DEF 和鸭感染 DHAV-1 后的变化。

Host miRNA and mRNA profiles during in DEF and duck after DHAV-1 infection.

机构信息

Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, China.

Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, China.

出版信息

Sci Rep. 2024 Sep 29;14(1):22575. doi: 10.1038/s41598-024-72992-x.

DOI:10.1038/s41598-024-72992-x

PMID:39343789

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11439951/

Abstract

DHAV-1 is a highly infectious pathogen that can cause acute hepatitis in ducklings. MicroRNA (miRNA) plays an essential regulatory role in virus response. We characterized and compared miRNA and mRNA expression profiles in duck embryonic fibroblasts (DEF) and the liver of ducklings infected with DHAV-1. DHAV-1 infected DEF was divided into infection group (D group) and blank group (M group), and DHAV-1 infected duckling group was divided into infection group (H group) and blank group (N group). D vs. M have 130 differentially expressed (DE) miRNA (DEM) and 2204 differentially expressed (DE) mRNA (DEG), H vs. N have 72 DEM and 1976 DEG. By the intersection of D vs. M and H vs. N comparisons, 15 upregulated DEM, 5 downregulated DEM, 340 upregulated DEG and 50 downregulated DEG were found with both in vivo and in vitro DHAV-1 infection. In particular, we identified the same DE miRNA target genes and functional annotations of DE mRNA. We enriched with multiple gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, which may have important roles in viral virulence, host immunity, and metabolism. We selected miR-155, which is co-upregulated, and found that miR-155 targets SOCS1 to inhibit DHVA-1 replication.

摘要

DHAV-1 是一种高度传染性病原体，可导致雏鸭急性肝炎。MicroRNA（miRNA）在病毒反应中发挥重要的调节作用。我们对感染 DHAV-1 的鸭胚成纤维细胞（DEF）和雏鸭肝脏中的 miRNA 和 mRNA 表达谱进行了特征分析和比较。DHAV-1 感染的 DEF 分为感染组（D 组）和空白组（M 组），DHAV-1 感染的雏鸭分为感染组（H 组）和空白组（N 组）。D 与 M 相比有 130 个差异表达（DE）miRNA（DEM）和 2204 个差异表达（DE）mRNA（DEG），H 与 N 相比有 72 个 DEM 和 1976 个 DEG。通过 D 与 M 和 H 与 N 的比较，在体内和体外 DHAV-1 感染中均发现 15 个上调的 DEM、5 个下调的 DEM、340 个上调的 DEG 和 50 个下调的 DEG。特别是，我们鉴定了具有相同功能注释的 DE miRNA 靶基因和 DE mRNA。我们对多个基因本体论（GO）和京都基因与基因组百科全书（KEGG）途径进行了富集，这些途径可能在病毒毒力、宿主免疫和代谢中具有重要作用。我们选择了共同上调的 miR-155，并发现 miR-155 靶向 SOCS1 抑制 DHVA-1 复制。