Metcalfe C D, Sonstegard R A
J Natl Cancer Inst. 1985 Dec;75(6):1091-7.
Extracts prepared from oil refinery effluents (soxhlet and XAD-2) were tested for carcinogenic potential by means of the rainbow trout (Salmo gairdneri) embryo-injection bioassay. No neoplasms were detected in fish given injections of refinery extracts alone (with and without exogenous rat S-9 activation). Refinery extracts coinjected with aflatoxin B1 induced elevated frequencies of hepatic neoplasms. This cocarcinogenic effect was most pronounced when aflatoxin B1 was preincubated with rat S-9 prior to injection. Effluent extracts coinjected with a direct-acting carcinogen [N-methyl-N'-nitro-N-nitrosoguanidine (CAS: 56-57-5)] did not increase the incidence of hepatic neoplasms (with and without exogenous S-9 activation).
通过虹鳟（Salmo gairdneri）胚胎注射生物测定法，对炼油厂废水提取物（索氏提取物和XAD - 2）的致癌潜力进行了测试。单独注射炼油厂提取物的鱼（无论有无外源性大鼠S - 9激活）均未检测到肿瘤。与黄曲霉毒素B1共同注射的炼油厂提取物会导致肝脏肿瘤的发生频率升高。当黄曲霉毒素B1在注射前与大鼠S - 9预孵育时，这种促癌作用最为明显。与直接作用致癌物[N - 甲基 - N' - 硝基 - N - 亚硝基胍（CAS：56 - 57 - 5）]共同注射的废水提取物并未增加肝脏肿瘤的发生率（无论有无外源性S - 9激活）。
