Metcalfe C D, Sonstegard R A
J Natl Cancer Inst. 1986 Dec;77(6):1299-302.
An increased incidence of anaphase aberrations was observed in the tissues of rainbow trout embryos (Salmo gairdneri) within 96 hours of exposure to mitomycin [(MM) CAS: 50-07-7; 50 and 100 ng/embryo] and aflatoxin B1 [(AFB1) CAS: 1162-65-8; 13 and 25 ng/embryo] with the use of the trout embryo microinjection assay. High numbers of anaphase aberrations in embryos exposed to MM were associated with low mitotic indices, cell pyknosis, and high embryo mortalities. When the anaphase aberration data for embryos exposed to AFB1 were compared to previously reported carcinogenesis data from the microinjection assay, the incidence of anaphase aberrations in embryos did not show the same quantitative variations as the incidence of hepatic carcinomas in adult trout. While anaphase aberrations may be an indicator of the clastogenic effects of chemicals on fish embryos, it is unlikely that these aberrations can be used as an early indicator of a carcinogenic response in the trout embryo assay.
在虹鳟鱼胚胎（虹鳟）组织中，使用虹鳟胚胎显微注射试验观察到，在暴露于丝裂霉素[（MM）化学物质登记号：50 - 07 - 7；50和100纳克/胚胎]和黄曲霉毒素B1[（AFB1）化学物质登记号：1162 - 65 - 8；13和25纳克/胚胎]的96小时内，后期畸变的发生率有所增加。暴露于MM的胚胎中大量的后期畸变与低有丝分裂指数、细胞固缩和高胚胎死亡率相关。当将暴露于AFB1的胚胎的后期畸变数据与先前报道的显微注射试验致癌数据进行比较时，胚胎中后期畸变的发生率与成年虹鳟肝癌的发生率并未表现出相同的定量变化。虽然后期畸变可能是化学物质对鱼类胚胎的致断裂效应的一个指标，但在虹鳟胚胎试验中，这些畸变不太可能被用作致癌反应的早期指标。
