Ruffmann R, Schlick E, Tartaris T, Gruys E, Welker R D, Saito T, Chirigos M A
Med Oncol Tumor Pharmacother. 1985;2(3):195-202. doi: 10.1007/BF02934548.
Tumor bearing patients have repeatedly been shown to develop states of protein (PD). PD has frequently been associated with depression of acquired immunity. In contrast, little is known about the effects of PD on natural killer (NK) cell cytotoxicity and on bone marrow cellularity and how modulation of these parameters by maleic anhydride divinyl ether copolymer (MVE-2) would be affected. BALB/c mice 6-10 weeks old were fed normal diets (18% protein) (ND mice) or protein deficient isocaloric diets (PD mice) for 35 days. On days 5, 9, 15, 23, and 35 assays were performed. Three days prior to each assay day, some groups received MVE-2 i.p. In PD mice spontaneous NK activity was reduced by some 80%. After MVE-2 the levels only rose to half the amount of activity of ND mice, although in both groups an increase of NK activity took place. BMC levels of PD mice were also strongly decreased. After MVE-2, increases of BMC in PD mice were quite weak and did not reach the level of normal unstimulated BMC. In PD mice we found body weight loss of 46%, reduction in albumin and total protein of 22% and complete disappearance of prealbumin after 5 days of diets. Preliminary results indicate that repletion with amino acids (NeoAminomel L12.5 o.K.H. Salvia, Boehringer Mannheim Co.) is able restore NK activities and BMC levels.
携带肿瘤的患者反复出现蛋白质缺乏（PD）状态。PD常与获得性免疫抑制相关。相比之下，关于PD对自然杀伤（NK）细胞细胞毒性、骨髓细胞数量的影响以及马来酸酐二乙烯基醚共聚物（MVE - 2）对这些参数的调节作用如何，人们了解甚少。给6 - 10周龄的BALB/c小鼠喂食正常饮食（18%蛋白质）（ND小鼠）或蛋白质缺乏的等热量饮食（PD小鼠）35天。在第5、9、15、23和35天进行检测。在每个检测日前三天，部分组腹腔注射MVE - 2。在PD小鼠中，自发NK活性降低了约80%。注射MVE - 2后，其水平仅升至ND小鼠活性的一半，尽管两组NK活性均有所增加。PD小鼠的骨髓细胞（BMC）水平也显著降低。注射MVE - 2后，PD小鼠BMC的增加非常微弱，未达到正常未刺激BMC的水平。在PD小鼠中，我们发现饮食5天后体重减轻46%，白蛋白和总蛋白减少22%，前白蛋白完全消失。初步结果表明，补充氨基酸（NeoAminomel L12.5 o.K.H. Salvia，Boehringer Mannheim公司）能够恢复NK活性和BMC水平。
