评估口腔黏膜下纤维化中表达癌症代谢酶（Cyp1a1和Gstm1）的基因多态性的重要性。

Evaluation of the Importance of Genetic Polymorphisms in Genes Expressing Cancer-Metabolizing Enzymes (Cyp1a1 and Gstm1) in Oral Submucous Fibrosis.

作者信息

Pradhan Devina, Mehta Tarang, Srivastava Arpita, Patel Deepak, Dash Kailash Chandra, Hittalamani Vidya, Makkad Ramanpal Singh

机构信息

Department of Public Health Dentistry, Sardar Patel Post Graduate Institute of Dental and Medical Sciences, Lucknow, Uttar Pradesh, India.

Department of Oral and Maxillofacial Pathology and Oral Microbiology, Narsinhbhai Patel Dental College and Hospital, Sankalchand Patel University, Gujarat, India.

出版信息

J Pharm Bioallied Sci. 2024 Jul;16(Suppl 3):S2785-S2787. doi: 10.4103/jpbs.jpbs_413_24. Epub 2024 Jun 7.

DOI:10.4103/jpbs.jpbs_413_24

PMID:39346171

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11426692/

Abstract

BACKGROUND

Genetic polymorphisms are common and contribute significantly to human illnesses.

AIM

This study was carried out to evaluate the importance of genetic variations in the genes expressing cancer-metabolizing enzymes (CYP1A1 and GSTM1) in individuals experiencing oral submucous fibrosis (OSMF).

METHODS AND MATERIALS

Based on the clinical and histological characteristics of OSMF, 40 patients were chosen for the study; 10 of these patients had considerable polymorphism and malignant transformation; therefore, they were placed in a different group. After receiving written agreement, 30 normal subject patients were also picked for the study. For both normal and OSMF patients, tissue samples and 2 ml of peripheral venous blood were drawn from the arm vein and placed in a heparinized test tube. Electrophoresis on 0.8% agarose gel was used to verify genomic DNA.

RESULTS

The GSTM1 polymorphism, CYP1A1 polymorphism was 10.41% and 15.27% in normal subjects. The GSTM1 polymorphsm, CYP1A1 polymorphism was 16.21% and 8.14% in early OSMF. The GSTM1 polymorphsm, CYP1A1 polymorphism was 20.31% and 28.56% in moderate OSMF. The GSTM1 polymorphsm, CYP1A1 polymorphism was 8.12% and 12.54% in moderate OSMF. Finally, GSTM1 polymorphism was 60.47% in OSMF+ CA, while CYP1A1 polymorphism was 40.21%. The GSTM1 gene polymorphism and CYP1A1 gene polymorphism were maximum in OSMF+ CA category.

CONCLUSION

There is a significant role of genetic variations in the genes expressing cancer-metabolizing enzymes (CYP1A1) and GSTM1 in individuals experiencing OSMF.

摘要

背景

基因多态性普遍存在，对人类疾病有重大影响。

目的

本研究旨在评估口腔黏膜下纤维化（OSMF）患者中表达癌症代谢酶的基因（CYP1A1和GSTM1）的基因变异的重要性。

方法和材料

根据OSMF的临床和组织学特征，选择40例患者进行研究；其中10例患者有明显的多态性和恶性转化，因此被分入不同组。在获得书面同意后，还挑选了30例正常受试者作为研究对象。对于正常和OSMF患者，从手臂静脉采集组织样本和2毫升外周静脉血，置于肝素化试管中。使用0.8%琼脂糖凝胶电泳验证基因组DNA。

结果

正常受试者中GSTM1多态性、CYP1A1多态性分别为10.41%和15.27%。早期OSMF中GSTM1多态性、CYP1A1多态性分别为16.21%和8.14%。中度OSMF中GSTM1多态性、CYP1A1多态性分别为20.31%和28.56%。晚期OSMF中GSTM1多态性、CYP1A1多态性分别为8.12%和12.54%。最后，OSMF+CA中GSTM1多态性为60.47%，而CYP1A1多态性为40.21%。OSMF+CA类别中GSTM1基因多态性和CYP1A1基因多态性最高。