Rathore Anushree, Tiwari Anushree, Nazim Maira, Gupta Anup Kumar, Gande Mounica, Krishnakumar Jyothi
Department of Oral Pathology and Microbiology, Bhabha College of Dental Sciences, Madhya Pradesh, India.
Research Analyst, American Academy of Orthopedic Surgeons, Chicago, IL, US.
J Pharm Bioallied Sci. 2022 Jul;14(Suppl 1):S820-S824. doi: 10.4103/jpbs.jpbs_9_22. Epub 2022 Jul 13.
This study was carried out with the objective of recognizing the involvement of human papillomavirus (HPV) in conditions of oral squamous cell carcinoma (OSCC) and oral potentially malignant conditions and lesions.
OSCC samples from thirty patients were collected and thirty paraffin-embedded, oral premalignant specimens were collected. It was decided to use as a negative control samples gingiva from healthy individuals who had no prior history of oral proliferative leukoplakia or OSCC. The samples were then processed using a paraffin-embedded tissue block method. Deoxyribonucleic acid will be extracted from the paraffin-embedded tumor sample of sixty case subjects and tested for the presence of HPV DNA using polymerase chain reaction.
In study Group 1, 24 samples were taken from males and 6 from females. In study Group 2, 26 cases were males and 4 were females. In Group 1, 16 cases of moderately differentiated and 14 cases of well differentiated each were present. No case of poorly differentiated were found. There was statistically no significant difference present between male and female when Group 1 and Group 2 was considered. In study Group 1, 0 samples had positive HPV DNA detection, whereas 30 samples had negative HPV DNA detection. In study Group 2, 0 samples had positive HPV DNA detection, whereas 30 samples had negative HPV expression.
According to our findings, certain incidences of OSCC may be linked to HPV, however, this was not the case for all of the cancers.
本研究旨在确定人乳头瘤病毒（HPV）在口腔鳞状细胞癌（OSCC）及口腔潜在恶性病变中的作用。
收集30例OSCC患者的样本以及30例石蜡包埋的口腔癌前病变标本。决定将无口腔增殖性白斑或OSCC病史的健康个体的牙龈组织作为阴性对照样本。然后采用石蜡包埋组织块法对样本进行处理。从60例受试者的石蜡包埋肿瘤样本中提取脱氧核糖核酸，并使用聚合酶链反应检测HPV DNA的存在情况。
研究组1中，24个样本取自男性，6个样本取自女性。研究组2中，26例为男性，4例为女性。研究组1中，有16例中度分化，14例高分化，未发现低分化病例。将研究组1和研究组2综合考虑时，男女之间在统计学上无显著差异。研究组1中，0个样本HPV DNA检测呈阳性，30个样本HPV DNA检测呈阴性。研究组2中，0个样本HPV检测呈阳性，30个样本HPV表达呈阴性。
根据我们的研究结果，某些OSCC病例可能与HPV有关，然而，并非所有癌症都是如此。
