Department of Orthopedics and Research Institute of Orthopedics, West China Hospital, Sichuan University, Chengdu, 610041, China.
Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, 610041, China.
Theranostics. 2024 Sep 16;14(15):6016-6035. doi: 10.7150/thno.98219. eCollection 2024.
Total hip arthroplasty (THA) and total knee arthroplasty (TKA) are effective interventions for end-stage osteoarthritis; however, periprosthetic infection is a devastating complication of arthroplasty. To safely prevent periprosthetic infection and enhance osteointegration, the surface modification strategy was utilized to kill bacteria, modulate the osteoimmune microenvironment, and improve new bone formation. We used the hydrothermal method to fabricate a bionic insect wing with the disordered titanium dioxide nanoneedle (TNN) coating. The mussel-inspired poly-dopamine (PDA) and antibacterial silver nanoparticles (AgNPs) were coated on TNN, named AgNPs-PDA@TNN, to improve the biocompatibility and long-lasting bactericidal capacity. The physicochemical properties of the engineered specimen were evaluated with SEM, AFM, XPS spectrum, and water contact assay. The biocompatibility, bactericidal ability, and the effects on macrophages and osteogenic differentiation were assessed with RT-qPCR, Western blotting, live/dead staining, immunofluorescent staining, . The AgNPs-PDA@TNN were biocompatible with macrophages and exhibited immunomodulatory ability to promote M2 macrophage polarization. In addition, AgNPs-PDA@TNN ameliorated the cytotoxicity caused by AgNPs, promoted cell spreading, and increased osteogenesis and matrix deposition of BMSCs. Furthermore, AgNPs-PDA@TNN exhibited bactericidal ability against and by the bionic nanostructure and coated AgNPs. Various imaging analyses indicated the enhanced bactericidal ability and improved new bone formation by AgNPs-PDA@TNN . H&E, Gram, and Masson staining, verified the improved bone formation, less inflammation, infection, and fibrosis encapsulation. The immunofluorescence staining confirmed the immunomodulatory ability of AgNPs-PDA@TNN . The bionic insect wing AgNPs-PDA@TNN coating exhibited bactericidal property, immunomodulatory ability, and enhanced osteointegration. Thus, this multidimensional bionic implant surface holds promise as a novel strategy to prevent periprosthetic infection.
全髋关节置换术（THA）和全膝关节置换术（TKA）是治疗终末期骨关节炎的有效方法；然而，假体周围感染是关节置换术的一种破坏性并发症。为了安全预防假体周围感染和增强骨整合，采用表面改性策略来杀灭细菌、调节骨免疫微环境和促进新骨形成。我们使用水热法制备了具有无序二氧化钛纳米针（TNN）涂层的仿生昆虫翅膀。贻贝启发的聚多巴胺（PDA）和抗菌银纳米颗粒（AgNPs）涂覆在 TNN 上，命名为 AgNPs-PDA@TNN，以提高生物相容性和持久的杀菌能力。用 SEM、AFM、XPS 谱和水接触试验评估了工程标本的物理化学性质。通过 RT-qPCR、Western blot、活/死染色、免疫荧光染色评估了生物相容性、杀菌能力以及对巨噬细胞和成骨分化的影响。AgNPs-PDA@TNN 与巨噬细胞具有生物相容性，并表现出免疫调节能力，可促进 M2 巨噬细胞极化。此外，AgNPs-PDA@TNN 减轻了 AgNPs 的细胞毒性，促进了细胞铺展，并增加了 BMSCs 的成骨和基质沉积。此外，AgNPs-PDA@TNN 具有仿生纳米结构和涂覆的 AgNPs 对 和 的杀菌能力。各种成像分析表明，AgNPs-PDA@TNN 具有增强的杀菌能力和改善的新骨形成。H&E、革兰氏和 Masson 染色证实了新骨形成的改善、炎症、感染和纤维化包封的减少。免疫荧光染色证实了 AgNPs-PDA@TNN 的免疫调节能力。仿生昆虫翅膀 AgNPs-PDA@TNN 涂层具有杀菌特性、免疫调节能力和增强的骨整合能力。因此，这种多维仿生植入物表面具有作为预防假体周围感染的新策略的潜力。
