Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, China.
Adv Healthc Mater. 2019 Mar;8(5):e1801465. doi: 10.1002/adhm.201801465. Epub 2019 Jan 23.
Antibacterial and osteogenic design is required for ideal orthopedic implants. The excellent antimicrobial performance of silver nanoparticles (AgNPs) has attracted interest for the treatment of implant-related infections. However, the dose-dependent cytotoxicity of silver and its negative impact on bone implants restrict the further use of AgNPs coatings. Therefore, a hybrid coating containing polydopamine (PDA), hydroxyapatite (HA), AgNPs, and chitosan (CS) is prepared. Organic chelators CS and PDA that have promising biocompatibility are used to prevent the rapid release of silver ions from the AgNPs coating. The double chelating effect of PDA and CS significantly reduces silver ion release from the hybrid coating. The coating exhibits excellent anti-biofilm efficiency of 91.7%, 89.5%, and 92.0% for Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli, respectively. In addition, the coating can significantly stimulate osteogenic differentiation of MC3T3-E1 cells and promote bone-implant osseointegration in vivo as compared to that in the control group. The longitudinal biosafety of the coating is confirmed in vivo by histological evaluation and blood tests. The results of this study indicate that the hybrid coating exhibits antibacterial properties as well as allow bone-implant osseointegration, thereby providing insight into the design of multifunctional implants for long-term orthopedic applications.
抗菌和促成骨设计是理想的骨科植入物所必需的。银纳米粒子（AgNPs）具有出色的抗菌性能，因此引起了人们对治疗与植入物相关感染的兴趣。然而，银的剂量依赖性细胞毒性及其对骨植入物的负面影响限制了 AgNPs 涂层的进一步应用。因此，制备了一种包含聚多巴胺（PDA）、羟基磷灰石（HA）、AgNPs 和壳聚糖（CS）的混合涂层。具有良好生物相容性的有机螯合剂 CS 和 PDA 被用于防止 AgNPs 涂层中银离子的快速释放。PDA 和 CS 的双重螯合作用显著降低了混合涂层中银离子的释放。与对照组相比，该涂层对金黄色葡萄球菌、表皮葡萄球菌和大肠杆菌的抗生物膜效率分别达到了 91.7%、89.5%和 92.0%。此外，该涂层可以显著刺激 MC3T3-E1 细胞的成骨分化，并促进体内骨-植入物的骨整合。通过组织学评价和血液检查证实了该涂层在体内的长期生物安全性。本研究结果表明，该混合涂层具有抗菌性能，同时允许骨-植入物骨整合，从而为长期骨科应用的多功能植入物的设计提供了思路。
