Arra Yugander, Loo Eliza P-I, Devanna B N, Stiebner Melissa, Frommer Wolf B
Faculty of Mathematics and Natural Sciences, Institute for Molecular Physiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram (IISER TVM), Maruthamala P.O., Vithura, Thiruvananthapuram-695551, Kerala, India.
Bio Protoc. 2024 Sep 20;14(18):e5069. doi: 10.21769/BioProtoc.5069.
Improving desirable traits of popular rice varieties is of particular importance for small-scale food producers. Breeding is considered the most ecological and economic approach to improve yield, especially in the context of pest and pathogen-resistant varieties development. Being able to cross rice lines is also a critical step when using current transgene-based genome editing technologies, e.g., to remove transgenes. Moreover, rice breeders have developed accelerated breeding methods, including marker-assisted backcross breeding (MABB) to develop novel rice varieties with in-built resistance to biotic and abiotic stressors, grain, and nutritional quality. MABB is a highly efficient and cost-effective approach in accelerating the improvement of recipient variety by introgressing desirable traits, especially from landrace cultivars and wild rice accessions. Here, we provide a detailed protocol including video instructions for rice crossing and MABB to introgress target trait(s) of interest into the elite rice line. Further, we also highlight tips and tricks to be considered for a successful crossing and MABB. Key features • This protocol provides detailed information on techniques for crossing rice varieties and for breeding rice varieties with new traits • The protocol includes instructions for making rice crosses as well as MABB • The protocol provides beginners with detailed instructions including troubleshooting guides Graphical overview Illustration of crosses of rice lines and marker-assisted backcross breeding (created with BioRender.com).
改良流行水稻品种的优良性状对小规模粮食生产者尤为重要。育种被认为是提高产量最生态和经济的方法，特别是在开发抗病虫害品种的背景下。在使用当前基于转基因的基因组编辑技术（例如去除转基因）时，能够使水稻品系杂交也是关键步骤。此外，水稻育种者已经开发出加速育种方法，包括标记辅助回交育种（MABB），以培育对生物和非生物胁迫、谷物及营养品质具有内在抗性的新型水稻品种。MABB是一种通过导入优良性状，特别是从地方品种和野生稻种质中导入优良性状，来加速受体品种改良的高效且经济有效的方法。在此，我们提供了一份详细的方案，包括水稻杂交和MABB的视频说明，以便将感兴趣的目标性状导入优良水稻品系。此外，我们还强调了成功进行杂交和MABB时应考虑的技巧。关键特性 • 本方案提供了有关水稻品种杂交技术和培育具有新性状水稻品种的详细信息 • 该方案包括水稻杂交以及MABB的说明 • 该方案为初学者提供了详细说明，包括故障排除指南 图形概述 水稻品系杂交和标记辅助回交育种的示意图（由BioRender.com创建）。
