关于人嗜酸性粒细胞体外形成白三烯C4的最佳条件。

On the optimal conditions of LTC4 formation by human eosinophils in vitro.

作者信息

Bruynzeel P L, Kok P T, Viëtor R J, Verhagen J

出版信息

Prostaglandins Leukot Med. 1985 Oct;20(1):11-22. doi: 10.1016/0262-1746(85)90090-3.

Abstract

The optimal conditions for the -in vitro- LTC4 formation by the human eosinophil, isolated from peripheral blood, have been investigated in detail. LTC4 formation was found strongly Ca2+ and ionophore dependent and was complete after 20 min. Maximal LTC4 production was observed in the presence of 2 mM Ca2+, 10 microM ionophore A23187 and 5 mM glutathione. Addition of arachidonic acid resulted in a significant inhibition of the LTC4-synthesis by human eosinophils. In contrast, the formation of 15-HETE was strongly stimulated by the addition of arachidonic acid. As the LTC4 synthesis was found to be strongly inhibited by the addition of 15(S)-HETE to the incubation medium, this monohydroxy acid may be responsible for the inhibitory activity of arachidonic acid.

摘要

已详细研究了从外周血分离的人嗜酸性粒细胞体外生成白三烯C4（LTC4）的最佳条件。发现LTC4的生成强烈依赖于Ca2+和离子载体，20分钟后反应完成。在2 mM Ca2+、10 μM离子载体A23187和5 mM谷胱甘肽存在的情况下观察到最大LTC4产量。添加花生四烯酸会导致人嗜酸性粒细胞LTC4合成受到显著抑制。相反，添加花生四烯酸会强烈刺激15-羟基二十碳四烯酸（15-HETE）的生成。由于发现向孵育培养基中添加15(S)-HETE会强烈抑制LTC4合成，这种单羟基酸可能是花生四烯酸抑制活性的原因。