Dasgupta Uddipan, Ghosh Malabika, Gangopadhyay Rupali, Anh Nguyen Thi Ngoc, Doong Ruey-An, Sadhukhan Provash Chandra, Dutta Chowdhury Ankan
Amity Institute of Nanotechnology, Amity University Kolkata, Major Arterial Road, AA II, Newtown, Kolkata 700135, West Bengal, India.
Department of Chemistry, Sister Nivedita University, Action Area I, DG Block, 1/2, New Town, Kolkata 700156, West Bengal, India.
ACS Omega. 2024 Sep 10;9(38):40051-40060. doi: 10.1021/acsomega.4c05937. eCollection 2024 Sep 24.
A combination of magnetic and noble metal nanoparticles (NPs) has recently emerged as a potential substance for rapid and sensitive immunosorbent assays. However, to make the assay an alternative method for Enzyme-linked immunosorbent assay, the individual role of each nanoparticle must be explored properly. In this work, an immunoassay has been proposed using two antibody-conjugated iron oxide nanoparticles (FeONPs) and gold-silver bimetallic nanoparticles (AuAgNPs) to enhance the sensitivity of virus detection by colorimetric TMB/HO signal amplification. A synergistic effect is monitored between FeONPs and AuAgNPs, which is explored for colorimetric virus detection. The sensor exploits the synergistic effect between the nanoparticles to successfully detect a wide range of dengue virus-like particle (DENV-LP) concentrations ranging from 10 to 100 pg/mL with a detection limit of up to 2.6 fg/mL. In the presence of a target DENV-LP, a sandwich-like structure is formed, which restricts the electron transfer and the associated synergistic effect between the nanoparticles, restricting the TMB oxidation process. Therefore, the synergistic effect is the key to the present work, which accounts for the enhanced rate of the enzymatic reaction on TMB and makes the current method of virus detection more sensitive and reliable compared to the others.
磁性和贵金属纳米颗粒（NPs）的组合最近已成为一种用于快速灵敏免疫吸附测定的潜在物质。然而，为使该测定成为酶联免疫吸附测定的替代方法，必须正确探究每种纳米颗粒的单独作用。在这项工作中，已提出一种免疫测定方法，使用两种抗体偶联的氧化铁纳米颗粒（FeONPs）和金银双金属纳米颗粒（AuAgNPs），通过比色TMB/H₂O₂信号放大来提高病毒检测的灵敏度。监测到FeONPs和AuAgNPs之间的协同效应，并将其用于比色病毒检测。该传感器利用纳米颗粒之间的协同效应，成功检测出浓度范围为10至100 pg/mL的多种登革热病毒样颗粒（DENV-LP），检测限高达2.6 fg/mL。在存在目标DENV-LP的情况下，形成一种夹心样结构，这限制了电子转移以及纳米颗粒之间相关的协同效应，从而限制了TMB氧化过程。因此，协同效应是本工作的关键，它解释了TMB上酶促反应的增强速率，并且使当前的病毒检测方法相比其他方法更灵敏可靠。
