Feldt-Rasmussen B, Dinesen B, Deckert M
Scand J Clin Lab Invest. 1985 Oct;45(6):539-44. doi: 10.3109/00365518509155256.
An enzyme linked immunoadsorbent assay for urinary albumin using commercially available reagents is described. The assay range is 2.5-120 micrograms/l. When samples are analysed in two standard dilutions, the assayable albumin concentration range is 2.5-240 mg/l, covering the clinical range from normoalbuminuria to overt clinical nephropathy. Intra-assay variation was 2.1% and interassay variation 8.3%. Recovery of added albumin to urine was 95%-106% and dilution of urine was linear. The correlation to urinary albumin determined by immunodiffusion was excellent (n = 80, r = 0.99). Intraindividual variation of the 24 h urine albumin excretion of different days was high in patients with incipient diabetic nephropathy (51.5%) and was only slightly reduced by taking the variation of creatinine excretion into account (39.5%). No correlation was found between albumin excretion, and HbA1c or urine glucose excretion, indicating that minor metabolic variations are not responsible for the huge intraindividual day-to-day variations of UalbV. The study shows that more than one UalbV measurement must be done before classifying patients into groups with or without incipient diabetic nephropathy.
本文描述了一种使用市售试剂检测尿白蛋白的酶联免疫吸附测定法。该测定范围为2.5 - 120微克/升。当样品以两种标准稀释度进行分析时，可测定的白蛋白浓度范围为2.5 - 240毫克/升，涵盖了从正常白蛋白尿到明显临床肾病的临床范围。批内变异为2.1%，批间变异为8.3%。向尿液中添加白蛋白的回收率为95% - 106%，尿液稀释呈线性。与通过免疫扩散法测定的尿白蛋白的相关性极佳（n = 80，r = 0.99）。在早期糖尿病肾病患者中，不同日期24小时尿白蛋白排泄的个体内变异较高（51.5%），考虑肌酐排泄变异后仅略有降低（39.5%）。未发现白蛋白排泄与糖化血红蛋白或尿糖排泄之间存在相关性，这表明微小的代谢变化并非导致尿白蛋白排泄个体内每日巨大变异的原因。该研究表明，在将患者分类为有或无早期糖尿病肾病组之前，必须进行不止一次的尿白蛋白排泄量测量。
