Serebrennikova Kseniya V, Komova Nadezhda S, Aybush Arseniy V, Zherdev Anatoly V, Dzantiev Boris B
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky prospect 33, 119071 Moscow, Russia.
N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Kosygin Street 4, 119991 Moscow, Russia.
Materials (Basel). 2023 Feb 9;16(4):1475. doi: 10.3390/ma16041475.
Surface-enhanced Raman scattering (SERS) is considered an efficient technique providing high sensitivity and fingerprint specificity for the detection of pesticide residues. Recent developments in SERS-based detection aim to create flexible plasmonic substrates that meet the requirements for non-destructive analysis of contaminants on curved surfaces by simply wrapping or wiping. Herein, we reported a flexible SERS substrate based on cellulose fiber (CF) modified with silver nanostructures (AgNS). A silver film was fabricated on the membrane surface with an in situ silver mirror reaction leading to the formation of a AgNS-CF substrate. Then, the substrate was decorated through in situ synthesis of raspberry-like silver nanostructures (rAgNS). The SERS performance of the prepared substrate was tested using 4-mercaptobenzoic acid (4-MBA) as a Raman probe and compared with that of the CF-based plasmonic substrates. The sensitivity of the rAgNS/AgNS-CF substrate was evaluated by determining the detection limit of 4-MBA and an analytical enhancement factor, which were 10 nM and ~10, respectively. Further, the proposed flexible rAgNS/AgNS-CF substrate was applied for SERS detection of malathion. The detection limit for malathion reached 0.15 mg/L, which meets the requirements about its maximum residue level in food. Thus, the characteristics of the rAgNS/AgNS-CF substrate demonstrate the potential of its application as a label-free and ready-to-use sensing platform for the SERS detection of trace hazardous substances.
表面增强拉曼散射（SERS）被认为是一种高效技术，可为农药残留检测提供高灵敏度和指纹特异性。基于SERS的检测技术的最新进展旨在制造灵活的等离子体基底，通过简单的包裹或擦拭来满足对曲面上污染物进行无损分析的要求。在此，我们报道了一种基于用银纳米结构（AgNS）修饰的纤维素纤维（CF）的柔性SERS基底。通过原位银镜反应在膜表面制备银膜，从而形成AgNS-CF基底。然后，通过原位合成覆盆子状银纳米结构（rAgNS）对该基底进行修饰。使用4-巯基苯甲酸（4-MBA）作为拉曼探针测试所制备基底的SERS性能，并与基于CF的等离子体基底进行比较。通过测定4-MBA的检测限和分析增强因子来评估rAgNS/AgNS-CF基底的灵敏度，其检测限和分析增强因子分别为10 nM和约10。此外，将所提出的柔性rAgNS/AgNS-CF基底应用于马拉硫磷的SERS检测。马拉硫磷的检测限达到0.15 mg/L，满足其在食品中最大残留限量的要求。因此，rAgNS/AgNS-CF基底的特性证明了其作为用于SERS检测痕量有害物质的无标记且即用型传感平台的应用潜力。
