ABTS 法测定腐植酸产品的抗氧化性能。
Antioxidant Features of Humic Products by ABTS Assay.
机构信息
Department of Agricultural Sciences, University of Naples Federico II, Naples, Italy.
Interdepartmental Research Centre of Nuclear Magnetic Resonance for the Environment, AgriFood and New Materials (CERMANU), University of Naples Federico II, Naples, Italy.
出版信息
Methods Mol Biol. 2025;2857:223-227. doi: 10.1007/978-1-0716-4128-6_19.
Oxidative stress plays a pivotal role in driving immunosenescence by disrupting cellular homeostasis and impairing immune function. Humic substances exhibit scavenging activity against reactive oxygen species (ROS), inhibit ROS generation via metal chelation, and modulate endogenous antioxidant enzyme activity. Additionally, humic substances display anti-inflammatory effects, further supporting cellular redox balance. Given their antioxidant activity, humic substances hold promise as natural compounds for mitigating oxidative stress-associated immunosenescence. Here we describe the evaluation of antioxidant capacities of humic products by ABTS spectrophotometric assay.
氧化应激通过破坏细胞内稳态和损害免疫功能，在驱动免疫衰老中起关键作用。腐殖质具有清除活性氧（ROS）的作用，通过螯合金属来抑制 ROS 的产生，并调节内源性抗氧化酶的活性。此外，腐殖质还具有抗炎作用，进一步支持细胞内的氧化还原平衡。鉴于其抗氧化活性，腐殖质有望成为减轻与氧化应激相关的免疫衰老的天然化合物。在这里，我们通过 ABTS 分光光度法描述了腐殖质产品抗氧化能力的评估。
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