State Key Laboratory of Black Soils Conservation and Utilization, Key Laboratory of Wetland Ecology and Environment, Heilongjiang Xingkai Lake Wetland Ecosystem National Observation and Research Station, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.
State Key Laboratory of Black Soils Conservation and Utilization, Key Laboratory of Wetland Ecology and Environment, Heilongjiang Xingkai Lake Wetland Ecosystem National Observation and Research Station, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Sci Total Environ. 2024 Dec 1;954:176586. doi: 10.1016/j.scitotenv.2024.176586. Epub 2024 Sep 29.
Soil bacteria are vital to regulate biogeochemical processes in wetlands, however, little is known about the patterns and mechanisms of soil bacterial re-organization during wetland restoration. Here, we used a space-for-time substitution approach and examined the ecological processes that drive soil bacterial assembly from cultivated to restored to natural wetlands. Results showed a decrease of soil bacterial α diversity and increase of bacterial community similarity and bacterial interaction (cooperation vs. competition) with years of restoration, which was dominantly influenced by deterministic processes. Identified bacterial keystone taxa (e.g. Variibacter, Acidibacter) with nutrient metabolism capacity exerted strong positive effect on bacterial interaction. Furthermore, changes of soil water condition and nutrient status showed dominantly direct positive effects on soil bacterial reassembly, while falling soil pH significantly promoted bacterial reassembly by increasing keystone taxa and bacterial interaction during wetland restoration. Overall, findings highlighted the crucial role of environmental filtering and its pathway in influencing keystone bacterial taxa that promotes the reassembly of bacterial community during wetland restoration. Our work thus provides a new crucial and timely insight for improving the management of soil bacterial community assembly within the plethora of current and future wetland restoration projects.
土壤细菌对于调节湿地的生物地球化学过程至关重要，但对于湿地恢复过程中土壤细菌重组的模式和机制知之甚少。在这里，我们使用时空替代方法，研究了从耕地到湿地恢复再到自然湿地驱动土壤细菌组装的生态过程。结果表明，随着恢复年限的增加，土壤细菌 α 多样性减少，细菌群落相似性增加，细菌相互作用（合作与竞争）增加，这主要受确定性过程的影响。具有养分代谢能力的鉴定细菌关键分类群（例如 Variibacter、Acidibacter）对细菌相互作用产生强烈的正向影响。此外，土壤水分条件和养分状况的变化对土壤细菌重组表现出明显的直接正向影响，而土壤 pH 值的下降通过增加关键分类群和细菌相互作用，在湿地恢复过程中显著促进了细菌重组。总的来说，研究结果强调了环境过滤及其途径在影响关键细菌分类群方面的关键作用，这促进了湿地恢复过程中细菌群落的重组。因此，我们的工作为当前和未来众多湿地恢复项目中土壤细菌群落组装的管理提供了一个新的重要而及时的见解。
