采用小分子精确识别技术从西洋野芫荽（L.）豪夫根中靶向分离木脂素。

Targeted isolation of lignans from the roots of Anthriscus sylvestris (L.) Hoffm. by small molecule accurate recognition technology.

机构信息

School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou, 450046, China; Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Disease Diagnosis by Henan and Education Ministry of PR China, Zhengzhou, 450046, China.

出版信息

Phytochemistry. 2025 Jan;229:114293. doi: 10.1016/j.phytochem.2024.114293. Epub 2024 Sep 28.

DOI:10.1016/j.phytochem.2024.114293

PMID:39349233

Abstract

Five undescribed lignans (1-5), along with sixteen known lignans (6-21), were isolated from the roots of Anthriscus sylvestris using small molecule accurate recognition technology (SMART). The structures of the isolated compounds were determined by comprehensive spectroscopic analyses, and the absolute configurations of compounds 3-5 were elucidated by comparison of their calculated and experimental ECD spectra. Compounds 5, 14-15, 19, and 21 exhibited significantly inhibitory effects against hypoxia-stimulated abnormal proliferation of pulmonary arterial smooth muscle cells (PASMCs). Moreover, compounds 5, 14-15, 19, and 21 can significantly restore expression of expression of PASMCs proliferation-related protein, including α-SMA, PCNA, P27, and CyclinD3, which are closely related to cell proliferation.

摘要

从欧洲野葛的根部分离得到了五个未被描述的木脂素（1-5）和十六个已知的木脂素（6-21），采用小分子精确识别技术（SMART）。通过全面的光谱分析确定了分离化合物的结构，并通过比较它们的计算和实验ECD 光谱确定了化合物 3-5 的绝对构型。化合物 5、14-15、19 和 21 对缺氧刺激的肺动脉平滑肌细胞（PASMCs）异常增殖具有显著的抑制作用。此外，化合物 5、14-15、19 和 21 可以显著恢复与细胞增殖密切相关的 PASMCs 增殖相关蛋白的表达，包括α-SMA、PCNA、P27 和 CyclinD3。