穗甘松香根中的二芳基戊烷类和苯丙素类化合物

Diarylpentanoids and phenylpropanoids from the roots of Anthriscus sylvestris (L.) Hoffm.

机构信息

School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province China, Zhengzhou, 450046, China; Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Disease Diagnosis by Henan and Education Ministry of PR China, Zhengzhou, 450046, China.

出版信息

Phytochemistry. 2023 Dec;216:113865. doi: 10.1016/j.phytochem.2023.113865. Epub 2023 Sep 22.

DOI:10.1016/j.phytochem.2023.113865

PMID:37743005

Abstract

Three pairs of undescribed diarylpentanoid enantiomers (1-3) and five undescribed phenylpropanoids (4-8), along with seven known compounds, were isolated from the roots of Anthriscus sylvestris. The structures of compounds (1-8) were determined by analysis of their 1D and 2D NMR spectra, HRESIMS, and electronic circular dichroism. In addition, the inhibitory activities against hypoxia-stimulated pulmonary arterial smooth muscle cells abnormal proliferation were evaluated by MTT assay. The mRNA expression levels of Bcl-2, BAX, Caspase3, and IL-6 were detected by quantitative real-time PCR. The results showed that compounds (-)-1, (+)-1, (-)-2, (+)-3, 4, 8-10, 14, and 15 inhibited the abnormal proliferation of PASMCs by regulating the levels of apoptosis and inflammatory factors.

摘要

从 Anthriscus sylvestris 的根部分离得到三对未描述的二芳基戊烷对映异构体（1-3）和五对未描述的苯丙素（4-8），以及七种已知化合物。通过分析它们的 1D 和 2D NMR 光谱、HRESIMS 和电子圆二色性，确定了化合物（1-8）的结构。此外，通过 MTT 测定评估了它们对缺氧刺激的肺动脉平滑肌细胞异常增殖的抑制活性。通过实时定量 PCR 检测 Bcl-2、BAX、Caspase3 和 IL-6 的 mRNA 表达水平。结果表明，化合物 (-)-1、(+)-1、(-)-2、(+)-3、4、8-10、14 和 15 通过调节细胞凋亡和炎症因子水平抑制 PASMC 的异常增殖。