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转录组分析与紫色稻米灌浆期抗氧化特性相关基因的共表达网络。

Transcriptome analysis of the coexpression network of genes related to antioxidant characteristics after grain filling in purple rice.

机构信息

Jiangsu Kingearth Seed Co., Ltd., Yangzhou, 225009, China.

Jiangsu Key Laboratory of Crop Genetics and Physiology, Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, 225009, China.

出版信息

Sci Rep. 2024 Sep 30;14(1):22612. doi: 10.1038/s41598-024-73698-w.

DOI:10.1038/s41598-024-73698-w
PMID:39349620
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11443096/
Abstract

Antioxidant capacity is an important indicator for evaluating the growth and developmental quality of rice. This study has guiding significance for the cultivation of high-nutrient-value varieties. To investigate the molecular mechanisms underlying the antioxidant characteristics of rice grains after the filling stage, Yangzinuo 1 (YZN1) was used as the experimental material, and grains collected at five different time points (7 days apart) after the filling stage were used for transcriptome sequencing. Through weighted gene coexpression network analysis (WGCNA), a coexpression network of gene weights related to antioxidant characteristics was constructed. LOC_Os10g39140, LOC_Os10g38276, and LOC_Os05g45740 were identified from the 2 modules showing the highest correlations with the target traits. GO functional annotation showed that target modules were enriched in pathways related to phenylalanine, flavonoids, and other related pathways, such as GO:0006558, GO:0006559, GO:0009812, and GO:0009813. Correlation analysis with metabolites revealed that differentially expressed genes were significantly enriched in pathways related to antioxidant characteristics and energy metabolism processes, such as glycolysis/gluconeogenesis and flavonoid biosynthesis. The core genes identified in this study were found to be highly correlated with antioxidant characteristics and enriched in pathways related to metabolic and energy pathways and molecular activities. These results provide an effective dataset supporting breeding targeting functional rice characteristics.

摘要

抗氧化能力是评估水稻生长和发育质量的重要指标。本研究对培育高营养价值品种具有指导意义。为了研究水稻灌浆后期谷粒抗氧化特性的分子机制，本研究以扬稻 1 号（YZN1）为实验材料，在灌浆后期每隔 7 天收集 5 个不同时间点的谷粒进行转录组测序。通过加权基因共表达网络分析（WGCNA），构建了与抗氧化特性相关的基因权重的共表达网络。从与目标性状相关性最高的 2 个模块中鉴定出 LOC_Os10g39140、LOC_Os10g38276 和 LOC_Os05g45740。GO 功能注释表明，目标模块富集在与苯丙氨酸、类黄酮等相关途径相关的途径中，如 GO:0006558、GO:0006559、GO:0009812 和 GO:0009813。与代谢物的相关性分析表明，差异表达基因在与抗氧化特性和能量代谢过程相关的途径中显著富集，如糖酵解/糖异生和类黄酮生物合成。本研究中鉴定的核心基因与抗氧化特性高度相关，并且在与代谢和能量途径以及分子活性相关的途径中富集。这些结果为针对功能水稻特性的选育提供了有效的数据集支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/17e88cb30136/41598_2024_73698_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/7368c2632e4d/41598_2024_73698_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/e4a348cee238/41598_2024_73698_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/3fc2151fe511/41598_2024_73698_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/1d5a7935559a/41598_2024_73698_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/17e88cb30136/41598_2024_73698_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/7368c2632e4d/41598_2024_73698_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/e4a348cee238/41598_2024_73698_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/3fc2151fe511/41598_2024_73698_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/1d5a7935559a/41598_2024_73698_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1368/11443096/17e88cb30136/41598_2024_73698_Fig5_HTML.jpg

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