• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

STAT1 作为一种非侵入性监测癌症中 NK 细胞激活的工具。

STAT1 as a tool for non-invasive monitoring of NK cell activation in cancer.

机构信息

Biopharmaceutical Research Center, Korea Basic Science Institute (KBSI), Cheongju, 28119, Republic of Korea.

Korea University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.

出版信息

Commun Biol. 2024 Sep 30;7(1):1222. doi: 10.1038/s42003-024-06917-9.

DOI:10.1038/s42003-024-06917-9
PMID:39349746
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11442705/
Abstract

Natural killer (NK) cells play a crucial role in immunotherapy for cancer due to their natural ability to target and destroy cancer cells. However, current methods to visualize NK cells' activity against tumors in live organisms are limited. We introduce an imaging method that non-invasively tracks NK cell activation by cancer cells through the STAT1 protein. To achieve this, we modified NK cells to include a specific genetic sequence that binds to STAT1 when activated. These engineered NK cells (GAS-NK) demonstrate their functionality through various biological tests and analysis. Observations of changes in cancer environments and patient-derived cancer organoid models further confirm the effectiveness of this approach. Our method provides a way to monitor NK cell activity, which could improve the prediction and effectiveness of NK cell-based cancer therapies, contributing to advances in cancer treatment.

摘要

自然杀伤 (NK) 细胞因其天然靶向和破坏癌细胞的能力，在癌症的免疫治疗中发挥着关键作用。然而，目前在活体生物中可视化 NK 细胞针对肿瘤活动的方法受到限制。我们介绍了一种成像方法，通过 STAT1 蛋白非侵入性地跟踪 NK 细胞对癌细胞的激活。为此，我们修改了 NK 细胞，使其包含一种特定的遗传序列，当被激活时与 STAT1 结合。这些工程化的 NK 细胞（GAS-NK）通过各种生物学测试和分析证明了它们的功能。对癌症环境变化和患者来源的癌症类器官模型的观察进一步证实了该方法的有效性。我们的方法提供了一种监测 NK 细胞活性的方法，这可以提高基于 NK 细胞的癌症治疗的预测和效果，有助于癌症治疗的进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/02752be3af83/42003_2024_6917_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/77d3a779d038/42003_2024_6917_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/40ec5ebfc87b/42003_2024_6917_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/6d18ec6b6152/42003_2024_6917_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/df833388caf1/42003_2024_6917_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/a7ed8f7115c9/42003_2024_6917_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/74a37933074b/42003_2024_6917_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/02752be3af83/42003_2024_6917_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/77d3a779d038/42003_2024_6917_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/40ec5ebfc87b/42003_2024_6917_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/6d18ec6b6152/42003_2024_6917_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/df833388caf1/42003_2024_6917_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/a7ed8f7115c9/42003_2024_6917_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/74a37933074b/42003_2024_6917_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47d1/11442705/02752be3af83/42003_2024_6917_Fig7_HTML.jpg

相似文献

1
STAT1 as a tool for non-invasive monitoring of NK cell activation in cancer.STAT1 作为一种非侵入性监测癌症中 NK 细胞激活的工具。
Commun Biol. 2024 Sep 30;7(1):1222. doi: 10.1038/s42003-024-06917-9.
2
Sensitization to immune checkpoint blockade through activation of a STAT1/NK axis in the tumor microenvironment.在肿瘤微环境中通过激活 STAT1/NK 轴实现免疫检查点阻断的敏化作用。
Sci Transl Med. 2019 Jul 17;11(501). doi: 10.1126/scitranslmed.aav7816.
3
NK Cell Metabolism and Tumor Microenvironment.自然杀伤细胞代谢与肿瘤微环境。
Front Immunol. 2019 Sep 24;10:2278. doi: 10.3389/fimmu.2019.02278. eCollection 2019.
4
Mechanisms of NK cell dysfunction in the tumor microenvironment and current clinical approaches to harness NK cell potential for immunotherapy.肿瘤微环境中 NK 细胞功能障碍的机制及当前利用 NK 细胞潜力进行免疫治疗的临床方法。
J Leukoc Biol. 2021 Jun;109(6):1071-1088. doi: 10.1002/JLB.5MR0920-198RR. Epub 2020 Sep 29.
5
Association of Germline Variants in Natural Killer Cells With Tumor Immune Microenvironment Subtypes, Tumor-Infiltrating Lymphocytes, Immunotherapy Response, Clinical Outcomes, and Cancer Risk.自然杀伤细胞种系变异与肿瘤免疫微环境亚型、肿瘤浸润淋巴细胞、免疫治疗反应、临床结局和癌症风险的关联。
JAMA Netw Open. 2019 Sep 4;2(9):e199292. doi: 10.1001/jamanetworkopen.2019.9292.
6
NK cells: An attractive candidate for cancer therapy.自然杀伤细胞：癌症治疗的有吸引力的候选者。
J Cell Physiol. 2019 Nov;234(11):19352-19365. doi: 10.1002/jcp.28657. Epub 2019 Apr 16.
7
Targeting NK Cell Checkpoint Receptors or Molecules for Cancer Immunotherapy.针对自然杀伤细胞检查点受体或分子的癌症免疫治疗。
Front Immunol. 2020 Jun 23;11:1295. doi: 10.3389/fimmu.2020.01295. eCollection 2020.
8
Natural Killer cells at the frontline in the fight against cancer.自然杀伤细胞在抗击癌症的第一线。
Cell Death Dis. 2024 Aug 23;15(8):614. doi: 10.1038/s41419-024-06976-0.
9
Engineering Natural Killer Cells for Cancer Immunotherapy.用于癌症免疫治疗的工程化自然杀伤细胞
Mol Ther. 2017 Aug 2;25(8):1769-1781. doi: 10.1016/j.ymthe.2017.06.012. Epub 2017 Jun 28.
10
The basic biology of NK cells and its application in tumor immunotherapy.自然杀伤细胞的基础生物学及其在肿瘤免疫治疗中的应用。
Front Immunol. 2024 Aug 16;15:1420205. doi: 10.3389/fimmu.2024.1420205. eCollection 2024.

引用本文的文献

1
Single‑cell RNA‑Seq reveals PBMC profile alterations in a patient following a radiation accident.单细胞RNA测序揭示了一名辐射事故患者外周血单核细胞谱的变化。
Exp Ther Med. 2025 Mar 14;29(5):96. doi: 10.3892/etm.2025.12846. eCollection 2025 May.

本文引用的文献

1
Immune Resistance Mechanisms and the Road to Personalized Immunotherapy.免疫抵抗机制与个体化免疫治疗之路。
Am Soc Clin Oncol Educ Book. 2023 Jun;43:e390290. doi: 10.1200/EDBK_390290.
2
Enhancing a Natural Killer: Modification of NK Cells for Cancer Immunotherapy.增强自然杀伤细胞：用于癌症免疫治疗的 NK 细胞修饰。
Cells. 2021 Apr 29;10(5):1058. doi: 10.3390/cells10051058.
3
Inability of granule polarization by NK cells defines tumor resistance and can be overcome by CAR or ADCC mediated targeting.NK 细胞的颗粒极化能力定义了肿瘤的耐药性，可通过 CAR 或 ADCC 介导的靶向作用来克服。
J Immunother Cancer. 2021 Jan;9(1). doi: 10.1136/jitc-2020-001334.
4
A Functional Chemiluminescent Probe for in Vivo Imaging of Natural Killer Cell Activity Against Tumours.用于体内成像自然杀伤细胞对肿瘤活性的功能化学发光探针。
Angew Chem Int Ed Engl. 2021 Mar 8;60(11):5699-5703. doi: 10.1002/anie.202011429. Epub 2021 Feb 2.
5
System-Based Approaches to Delineate the Antiviral Innate Immune Landscape.基于系统的方法来描绘抗病毒先天免疫景观。
Viruses. 2020 Oct 21;12(10):1196. doi: 10.3390/v12101196.
6
The immune contexture and Immunoscore in cancer prognosis and therapeutic efficacy.肿瘤免疫微环境与免疫评分在癌症预后和治疗疗效中的作用
Nat Rev Cancer. 2020 Nov;20(11):662-680. doi: 10.1038/s41568-020-0285-7. Epub 2020 Aug 4.
7
Application of In Vivo Imaging Techniques for Monitoring Natural Killer Cell Migration and Tumor Infiltration.体内成像技术在监测自然杀伤细胞迁移和肿瘤浸润中的应用。
Cancers (Basel). 2020 May 22;12(5):1318. doi: 10.3390/cancers12051318.
8
Harnessing natural killer cells for the treatment of ovarian cancer.利用自然杀伤细胞治疗卵巢癌。
Gynecol Oncol. 2020 Jun;157(3):810-816. doi: 10.1016/j.ygyno.2020.03.020. Epub 2020 Apr 5.
9
Recent progress in and challenges in cellular therapy using NK cells for hematological malignancies.NK 细胞用于血液系统恶性肿瘤的细胞治疗的最新进展和挑战。
Blood Rev. 2020 Nov;44:100678. doi: 10.1016/j.blre.2020.100678. Epub 2020 Mar 20.
10
Characterizing the Dysfunctional NK Cell: Assessing the Clinical Relevance of Exhaustion, Anergy, and Senescence.表征功能失调的自然杀伤细胞：评估耗竭、无反应性和衰老的临床相关性。
Front Cell Infect Microbiol. 2020 Feb 13;10:49. doi: 10.3389/fcimb.2020.00049. eCollection 2020.