College of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Henan Province Engineering Research Centers of Horticultural Plant Resource Utilization and Germplasm Enhancement, Xinxiang, 453003, China.
BMC Plant Biol. 2024 Oct 1;24(1):911. doi: 10.1186/s12870-024-05597-1.
The β-1,3-glucanase gene is widely involved in plant development and stress defense. However, an identification and expression analysis of the grape β-1,3-glucanase gene (VviBG) family had not been conducted prior to this study.
Here, 42 VviBGs were identified in grapevine, all of which contain a GH-17 domain and a variable C-terminal domain. VviBGs were divided into three clades α, β and γ, and six subgroups A-F, with relatively conserved motifs/domains and intron/exon structures within each subgroup. The VviBG gene family contained four tandem repeat gene clusters. There were intra-species synteny relationships between two pairs of VviBGs and inter-species synteny relationships between 20 pairs of VviBGs and AtBGs. The VviBG promoter contained many cis-acting elements related to stress and hormone responses. Tissue-specific analysis showed that VviBGs exhibited distinct spatial and temporal expression patterns. Transcriptome analysis indicated that many VviBGs were induced by wounds, UV, downy mildew, cold, salt and drought, especially eight VviBGs in subgroup A of the γ clade. RT-qPCR analysis showed that these eight VviBGs were induced under abiotic stress (except for VviBG41 under cold stress), and most of them were induced at higher expression levels by PEG6000 and NaCl than under cold treatment.
The chromosome localization, synteny and phylogenetic analysis of the VviBG members were first conducted. The cis-acting elements, transcriptome data and RT-qPCR analysis showed that VviBG genes play a crucial role in grape growth and stress (hormone, biotic and abiotic) responses. Our study laid a foundation for understanding their functions in grape resistance to different stresses.
β-1,3-葡聚糖酶基因广泛参与植物发育和应激防御。然而，在本研究之前，葡萄的β-1,3-葡聚糖酶基因（VviBG）家族尚未被鉴定和表达分析。
在此，在葡萄中鉴定出 42 个 VviBG，它们都含有 GH-17 结构域和可变的 C 端结构域。VviBG 分为三个分支 α、β和γ，以及六个亚组 A-F，每个亚组内都具有相对保守的基序/结构域和内含子/外显子结构。VviBG 基因家族包含四个串联重复基因簇。VviBG 基因在种内存在两个对之间的同线性关系，以及在 20 对 VviBG 和 AtBG 之间存在种间同线性关系。VviBG 启动子包含许多与应激和激素反应相关的顺式作用元件。组织特异性分析表明，VviBG 表现出不同的空间和时间表达模式。转录组分析表明，许多 VviBG 受到创伤、紫外线、霜霉病、寒冷、盐和干旱的诱导，尤其是γ分支亚组 A 中的 8 个 VviBG。RT-qPCR 分析表明，这些 8 个 VviBG 在非生物胁迫下被诱导（冷胁迫下除外 VviBG41），并且它们大多数在 PEG6000 和 NaCl 处理下的诱导表达水平高于冷处理。
首次对 VviBG 成员进行了染色体定位、同线性和系统发育分析。顺式作用元件、转录组数据和 RT-qPCR 分析表明，VviBG 基因在葡萄生长和应激（激素、生物和非生物）反应中发挥着关键作用。我们的研究为理解它们在葡萄对不同胁迫的抗性中的功能奠定了基础。
