ZYG11B 参与调节结直肠癌细胞增殖和免疫浸润，是一种预后生物标志物。

ZYG11B participates in the modulation of colorectal cancer cell proliferation and immune infiltration and is a prognostic biomarker.

机构信息

Department of Gastroenterology, Joint Logistic Support Force of PLA, 962 Hospital, Harbin City, 150080, China.

Laboratory of Cancer Biomarkers and Liquid Biopsy, School of Pharmacy, Henan University, Kaifeng, Henan, China.

出版信息

BMC Cancer. 2024 Sep 30;24(1):1203. doi: 10.1186/s12885-024-12963-7.

DOI:10.1186/s12885-024-12963-7

PMID:39350118

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11440697/

Abstract

PROPOSE

The biological function of ZYG11B is still unclear, and few studies on ZYG11B in colorectal cancer were reported. The purpose of our research is to detect the biological functions of ZYG11B in colorectal cancer through The Cancer Genome Atlas (TCGA) database online and the vitro cell experiments.

METHODS

The information of ZYG11B in colorectal cancer were downloaded from TCGA database. The bioinformatics analysis has been utilized to examine the expression, functional enrichment, association of immune, clinicopathological characteristics and diagnostic prognostic value. CCK-8 and apoptosis assays have been utilized to identify the abilities of progression and apoptosis. The abilities of invasion and migration were detected by transwell assay.

RESULTS

In contrast to adjacent normal tissues, colorectal cancer tissues exhibited a notably diminished expression of ZYG11B (p < 0.001). Further examination through functional enrichment analysis unveiled the enrichment of various pathways associated with tumor proliferation and apoptosis. The implementation of CCK-8 and apoptosis assays validated the suppressive impact of ZYG11B on the progression of colorectal cancer cells (p < 0.001). A significant positive correlation was found between ZYG11B and Tcm and T helper cells (R ≥ 0.3, p < 0.001). Moreover, the expression of ZYG11B demonstrated a prominent presence among subjects without previous experience of colon polyps (p < 0.05), devoid of lymphatic infiltration (p < 0.01), and age ≤ 65 years (p < 0.01). Additionally, ZYG11B exhibited higher expression levels among patients diagnosed with colorectal adenocarcinoma (p < 0.05). Following the analysis of survival prognosis, it became evident that increased ZYG11B expression correlated with enhanced survival rates (p < 0.01) and the ability to accurately forecast the prognosis and survival of COAD/READ patients.

CONCLUSION

ZYG11B plays a tumor suppressive role in the proliferation process of colorectal cancer and may have a broad application prospect in the diagnosis and prognosis evaluation of colorectal cancer with more study.

摘要

建议

ZYG11B 的生物学功能尚不清楚，关于结直肠癌中 ZYG11B 的研究较少。我们的研究目的是通过癌症基因组图谱 (TCGA) 数据库在线和体外细胞实验来检测 ZYG11B 在结直肠癌中的生物学功能。

方法

从 TCGA 数据库下载结直肠癌中 ZYG11B 的信息。利用生物信息学分析方法检测其表达、功能富集、与免疫的相关性、临床病理特征及诊断预后价值。CCK-8 及凋亡实验用于鉴定进展和凋亡能力。通过 Transwell 实验检测侵袭和迁移能力。

结果

与邻近正常组织相比，结直肠癌组织中 ZYG11B 的表达明显降低（p<0.001）。通过功能富集分析进一步研究揭示了与肿瘤增殖和凋亡相关的各种途径的富集。CCK-8 及凋亡实验验证了 ZYG11B 对结直肠癌细胞进展的抑制作用（p<0.001）。ZYG11B 与 Tcm 和 T 辅助细胞呈显著正相关（R≥0.3，p<0.001）。此外，在无结肠息肉病史的患者中 ZYG11B 的表达更为明显（p<0.05），无淋巴浸润（p<0.01），年龄≤65 岁（p<0.01）。此外，在诊断为结直肠腺癌的患者中 ZYG11B 表达水平较高（p<0.05）。对生存预后进行分析后发现，ZYG11B 表达增加与生存率提高相关（p<0.01），能够准确预测 COAD/READ 患者的预后和生存情况。