Zhao Xutao, Zan Lingxiong, He Niaofei, Liu Haidong, Xing Xiaorong, Du Dezhi, Tang Guoyong, Li Kaixiang
Academy of Agricultural and Forestry Sciences, Qinghai University, Qinghai-Tibet Plateau Germplasm Resources Research and Utilization Laboratory, Xining, 810016 Qinghai China.
Qinghai Key Laboratory for Genetic Improvement of Spring Rapeseed, Qinghai Branch of National Rapeseed Improvement Center, Xining, 810016 Qinghai China.
Mol Breed. 2024 Sep 30;44(10):68. doi: 10.1007/s11032-024-01503-7. eCollection 2024 Oct.
Determinate inflorescence is indeed a pivotal agricultural characteristic in crops, notably impacting the architecture modification of (AACC, 2n = 38). Previous study identified a crucial gene that encodes the transcription factor (). Here by two alleles were cloned and sequenced from indeterminate 2982 and determinate 4769, respectively, we found that harbors two T/C and G/C non-synonymous mutations in exon 1, and contains sixty-six differences in a 1.9 Kb promoter sequence. Subsequently, was introduced into 571 line by genetic complementation and overexpression, transgenic plants 571 lines and 571lines were all restored to the indeterminate inflorescence. Interestingly, after was knocked out in 'Westar', transgenic plants Westar lines were mutated to determinate inflorescences. Additionally, a NIL-4769 line was constructed to evaluate the effect of on agronomic traits of , the results demonstrated that reduced the plant height and increased the branch number and branch thousand grain weight of Finally, we performed RT-qPCR, GUS staining and subcellular localization experiments to analyze the expression pattern of , the results showed that the expression of at shoot apex of NIL-4769 was higher than that of 4769, GUS activity was detected at apical of and was detected in cell membrane, nucleus and cytoplasm. Our findings provide a firm molecular foundation for the study of rapeseed's molecular mechanism of determinate inflorescence formation, as well as theoretical guidance for the application of determinate inflorescence in rapeseed breeding.
The online version contains supplementary material available at 10.1007/s11032-024-01503-7.
有限花序确实是作物中一个关键的农艺性状，对油菜（AACC，2n = 38）的株型改良有显著影响。先前的研究鉴定出一个关键基因，该基因编码转录因子（）。在此，我们分别从无限花序的2982和有限花序的4769中克隆并测序了两个等位基因，发现该基因在第1外显子中有两个T/C和G/C非同义突变，并且在1.9 Kb的启动子序列中有66个差异。随后，通过遗传互补和过表达将该基因导入571品系，转基因植株571品系和571品系均恢复为无限花序。有趣的是，在‘Westar’中敲除该基因后，转基因植株Westar品系突变为有限花序。此外，构建了一个NIL - 4769品系来评估该基因对油菜农艺性状的影响，结果表明该基因降低了油菜的株高，增加了分枝数和分枝千粒重。最后，我们进行了RT - qPCR、GUS染色和亚细胞定位实验来分析该基因的表达模式，结果表明NIL - 4769茎尖中该基因的表达高于4769，在茎尖检测到GUS活性，并且在细胞膜、细胞核和细胞质中检测到该基因。我们的研究结果为研究油菜有限花序形成的分子机制提供了坚实的分子基础，也为有限花序在油菜育种中的应用提供了理论指导。
在线版本包含可在10.1007/s11032 - 024 - 01503 - 7获取的补充材料。
