探索与植物的有限花序和头状花序相关的自然变异基因。

Exploration into Natural Variation Genes Associated with Determinate and Capitulum-like Inflorescence in .

机构信息

Agricultural College, Guizhou University, Guiyang 550004, China.

Guizhou Rapeseed Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China.

出版信息

Int J Mol Sci. 2023 Aug 17;24(16):12902. doi: 10.3390/ijms241612902.

DOI:10.3390/ijms241612902

PMID:37629083

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10454214/

Abstract

is a globally important vegetable and oil crop. The research is meaningful for the yield and plant architecture of . In this study, one natural mutant line with determinate and capitulum-like inflorescence was chosen for further study. Genetic analysis indicated that the segregation patterns of inflorescences in the F populations supported a digenic inheritance model, which was further approved via the BSA-Seq technique. The BSA-Seq method detected two QTL regions on C02 (14.27-18.41 Mb) and C06 (32.98-33.68 Mb) for the genetic control of determinate inflorescences in MT plants. In addition, the expression profile in MT compared with WT was analyzed, and a total of 133 candidate genes for regulating the flower development (75 genes, 56.4%), shoot meristem development (29 genes, 21.8%), and inflorescence meristem development (13 genes, 9.8%) were identified. Then one joint analysis combing BSA-Seq and RNA-Seq identified two candidate genes of and for regulating the MT phenotype. Furthermore, the potential utilization of the MT plants was also discussed.

摘要

是一种重要的蔬菜和油料作物。该研究对于的产量和植物结构具有重要意义。本研究选择了一个具有定头状花序特性的自然突变体进行进一步研究。遗传分析表明，F1 群体的花序分离模式支持双基因遗传模型，该模型进一步通过 BSA-Seq 技术得到了证实。BSA-Seq 方法检测到两个与 C02（14.27-18.41 Mb）和 C06（32.98-33.68 Mb）上的决定花序的 QTL 区域，用于 MT 植物中定头状花序的遗传控制。此外，对 MT 与 WT 相比的表达谱进行了分析，共鉴定出 133 个候选基因，用于调节花发育（75 个基因，56.4%）、茎分生组织发育（29 个基因，21.8%）和花序分生组织发育（13 个基因，9.8%）。然后，通过结合 BSA-Seq 和 RNA-Seq 的联合分析，鉴定出和两个候选基因，用于调控 MT 表型。此外，还讨论了 MT 植物的潜在利用价值。