Research School of Polymeric Materials, School of Material Science & Engineering, Jiangsu University, Zhenjiang, 202113, China.
Jiangsu Biosurf Biotech Co., Ltd, Suzhou, 215123, P. R. China.
J Mater Chem B. 2024 Oct 30;12(42):10893-10901. doi: 10.1039/d4tb01780c.
As typical examples of pathological biomineralization, urinary stones and stent encrustation have been associated with bacteria, yet the underlying mechanisms remain unclear. In this study, the effect of Gram-negative and Gram-positive on the nucleation and growth of calcium oxalate crystals both in solution and on material surfaces was investigated. Both bacteria can promote calcium oxalate crystallization, and shows a prominent ability to boost the nucleation and growth rate. Interestingly, we discovered an Ostwald ripening phenomenon after the initial nucleation on the material surfaces, where larger particles emerge upon the disappearance of small nuclei particles, evident in the case of . Over an extended period of time, erosion and disintegration of the crystals was observed when bacteria were involved. Based on these understandings, we developed a new functional surface by synthesizing an antibacterial polypeptoid in-house and utilizing polyurethane as the substrate material. This surface exhibits a synergistic effect that inhibits the formation of calcium oxalate crystals. This study helps to elucidate the role of bacteria in calcium oxalate biomineralization and supports further development of treatment approaches such as anti-encrustation polymer materials.
作为病理性生物矿化的典型例子，尿路结石和支架上的结壳与细菌有关，但潜在的机制仍不清楚。在这项研究中，研究了革兰氏阴性菌和革兰氏阳性菌对溶液中和材料表面上草酸钙晶体成核和生长的影响。这两种细菌都能促进草酸钙结晶，而 表现出显著促进成核和生长速率的能力。有趣的是，我们在材料表面上的初始成核后发现了奥斯特瓦尔德熟化现象，其中较大的颗粒在小核颗粒消失时出现，在 的情况下尤为明显。在细菌存在的情况下，随着时间的延长，观察到晶体的侵蚀和崩解。基于这些认识，我们通过合成一种内源性的抗菌多肽和利用聚氨酯作为基底材料，开发了一种新的功能表面。该表面表现出协同抑制草酸钙晶体形成的作用。本研究有助于阐明细菌在草酸钙生物矿化中的作用，并支持开发抗结壳聚合物材料等治疗方法。
