Fang Beibei, Liu Qing, Yang Chengli, Liu Kaixuan, He Guanya, Zhai Xuchun, Hu Kaishun, Li Dali
Department of Bioengineering, School of Environmental & Biological Engineering, Nanjing University of Science & Technology, Nanjing, China.
Nat Prod Res. 2024 Oct 1:1-4. doi: 10.1080/14786419.2024.2411363.
A natural deep eutectic solvent-based ultrasound-assisted simultaneous extraction (NADES-UAE) of camptothecin (CPT) and 10-hydroxycamptothecin (10-HCPT) was established. The 1.31 mg of CPT and 1.66 mg of 10-HCPT were obtained from each gram of the fruit powder of under the optimum conditions with a water content of 20%, a liquid-solid ratio of 12 mL/g and an ultrasound time of 20 min. The recovery efficiencies of CPT and 10-HCPT after AB-8 resin enrichment were 70.5% and 74.8%, respectively. The stronger interaction between NADES3 which was screened from 12 kinds of NADES and target components compared with methanol or water was demonstrated using molecular dynamics simulation. Moreover, the recovered NADES3 could be reused at least 4 times. The present research provided an efficient, environment-friendly, and sustainable method for extracting and recovering CPT and 10-HCPT from the fruits of .
建立了一种基于天然深共熔溶剂的超声辅助同时提取喜树碱（CPT）和10-羟基喜树碱（10-HCPT）的方法（NADES-UAE）。在最佳条件下，即含水量20%、液固比12 mL/g、超声时间20分钟，每克果实粉末可获得1.31毫克CPT和1.66毫克10-HCPT。经AB-8树脂富集后，CPT和10-HCPT的回收率分别为70.5%和74.8%。通过分子动力学模拟表明，从12种NADES中筛选出的NADES3与目标成分之间的相互作用比甲醇或水更强。此外，回收的NADES3至少可重复使用4次。本研究为从果实中提取和回收CPT和10-HCPT提供了一种高效、环保且可持续的方法。
