Li Chunying, Zhang Yukun, Zhao Chunjian, Ni Yujiao, Wang Kaiting, Zhang Jingjing, Zhao Wenyan
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Collaborative Innovation Center for Development and Utilization of Forest Resources, Harbin 150040, China.
Molecules. 2017 Jun 27;22(7):1076. doi: 10.3390/molecules22071076.
A novel and efficient ultrasonic assisted-reflux synergistic extraction (UARSE) method for extracting camptothecin (CPT) and betulinic acid (BA) from Decne. fruits has been developed in this study. The advantages of the ultrasonic and reflux extraction methods have been combined in the UARSE method and used to extract CPT and BA for the first time. The parameters influencing the efficiency of UARSE were optimized using the Box-Behnken design (BBD) to obtain the maximum extraction yield of CPT and BA. The optimal extraction conditions were as follows: 225 W for the ultrasonic power; 24 min for the extraction time; and 32 mL/g for the liquid-solid ratio. The extraction yields obtained by UARSE were 2.386 ± 0.112 mg/g for CPT and 17.192 ± 0.808 mg/g for BA, which were 1.43-fold and 1.33-fold, respectively, higher than by using heating reflux extraction (HRE) and ultrasonic-assisted extraction (UAE). In addition, the 24-min extraction time using UARSE was 80% and 60% less than those provided by HRE and UAE, respectively. Therefore, UARSE can be considered a rapid and efficient method for extracting CPT and BA from the fruits of Decne.
本研究开发了一种新颖高效的超声辅助-回流协同萃取（UARSE）方法，用于从Decne.果实中提取喜树碱（CPT）和桦木酸（BA）。UARSE方法结合了超声和回流萃取方法的优点，并首次用于提取CPT和BA。采用Box-Behnken设计（BBD）对影响UARSE效率的参数进行优化，以获得CPT和BA的最大提取率。最佳提取条件如下：超声功率225W；提取时间24min；液固比32mL/g。UARSE获得的提取率分别为CPT 2.386±0.112mg/g和BA 17.192±0.808mg/g，分别比加热回流萃取（HRE）和超声辅助萃取（UAE）高1.43倍和1.33倍。此外，使用UARSE的24分钟提取时间分别比HRE和UAE提供的提取时间少80%和60%。因此，UARSE可被认为是一种从Decne.果实中提取CPT和BA的快速高效方法。
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