Parmar Kusum, Aggarwal Gaurav, Sharma Upendra
School of Pharmaceutical and Health Sciences, Career Point University, Hamirpur, Himachal Pradesh, 176 041, India.
Chemical Technology Division and AcSIR, CSIR-IHBT, Palampur, Himachal Pradesh, 176 061, India.
Chem Biodivers. 2025 Jan;22(1):e202401324. doi: 10.1002/cbdv.202401324. Epub 2024 Nov 4.
This work employed a green approach utilizing natural deep eutectic solvent (NADES)-assisted hydrodistillation for EO extraction from the aerial part of Ageratum conyzoides. Out of seven deep eutectic combinations used, glycerol-lactic acid (GLY:LA) (1 : 1) mixture significantly enhanced the yield from 0.78 mg/g (water as extraction media) to 1.00 mg/g. GC and GC-MS analysis revealed that EOs mainly contain (E)-β-caryophyllene (15.2-25.3 %), (E)-β-farnesene (2.7-8.2 %), and notably, precocene-I (8.2-18.0 %) and precocene-II (31.8-51.4 %), which varied significantly across different extraction media. Further column chromatography-based purification of EO led to the isolation of two known chromene derivatives precocene-I (1) and precocene-II (2). Precocene-I exhibited potent anti-diabetic activity (IC 0.26 mg/mL) compared to the standard drug acarbose. Among the EO samples, USK-N7, which had the highest percentage of precocene-I, showed the highest activity. The present study demonstrated the potential use of this weed plant as an anti-diabetic agent.
本研究采用绿色方法，利用天然低共熔溶剂（NADES）辅助水蒸馏法从藿香蓟地上部分提取挥发油（EO）。在所使用的七种低共熔组合中，甘油-乳酸（GLY:LA）（1:1）混合物显著提高了产率，从0.78毫克/克（以水作为提取介质）提高到1.00毫克/克。气相色谱（GC）和气相色谱-质谱联用（GC-MS）分析表明，挥发油主要含有（E）-β-石竹烯（15.2-25.3%）、（E）-β-法呢烯（2.7-8.2%），值得注意的是，早熟素-I（8.2-18.0%）和早熟素-II（31.8-51.4%），它们在不同提取介质中的含量差异显著。进一步通过柱色谱法对挥发油进行纯化，分离出两种已知的色烯衍生物早熟素-I（1）和早熟素-II（2）。与标准药物阿卡波糖相比，早熟素-I表现出较强的抗糖尿病活性（IC 0.26毫克/毫升）。在挥发油样品中，早熟素-I含量最高的USK-N7表现出最高活性。本研究证明了这种杂草植物作为抗糖尿病药物的潜在用途。
