Paperdrop Diagnostics S.L, MRB, Campus UAB, 08193, Bellaterra, Spain.
Nanobioelectronics and Biosensors Group, CSIC and BIST, Catalan Institute of Nanoscience and Nanotechnology (ICN2), Campus UAB, 08193, Bellaterra, Barcelona, Spain.
Mikrochim Acta. 2024 Oct 1;191(10):638. doi: 10.1007/s00604-024-06701-w.
Mainly performed within a rapid diagnostic tests company, a lateral flow (LF) system using gold nanoparticles (AuNPs) as transducers is presented able to detect three bacteria of interest, of relevance for antimicrobial resistance (AMR): Clostridioides difficile, methicillin-resistant Staphylococcus aureus (MRSA), and Klebsiella pneumoniae, with a limit of detection of 25 ng/mL of glutamate dehydrogenase (GDH) for C. difficile, 36 ng/mL of penicillin-binding protein 2a (PBP2a) for MRSA, and 4 × 10 CFU/mL for K. pneumoniae. The system showed good results with bacteria culture samples, is user-friendly, and suitable for rapid testing, as the results are obtained within 15 min.
本文介绍了一种侧向流（LF）系统，该系统主要在快速诊断测试公司中使用金纳米粒子（AuNPs）作为传感器，能够检测三种与抗菌药物耐药性（AMR）相关的感兴趣细菌：艰难梭菌、耐甲氧西林金黄色葡萄球菌（MRSA）和肺炎克雷伯菌，其谷氨酸脱氢酶（GDH）检测限分别为艰难梭菌 25ng/mL、MRSA 36ng/mL 和肺炎克雷伯菌 4×10CFU/mL。该系统在细菌培养样本中表现出良好的结果，易于使用，适合快速检测，因为结果可在 15 分钟内获得。
