Vocational School of Health Services, Bursa Uludag University, 16059, Bursa, Türkiye.
Department of Biology, Science and Art Faculty, Bursa Uludag University, 16059, Bursa, Türkiye.
Mol Biol Rep. 2024 Oct 1;51(1):1030. doi: 10.1007/s11033-024-09958-9.
The most prevalent malignancy among women is breast cancer (BC). MicroRNAs (miRNAs) play a role in the initiation and progression of BC by influencing breast cancer stem cells (BCSCs) but the diagnostic and prognostic roles of those miRNAs on BC patients are still unknown. It was aimed to investigate expression profiles, diagnostic and prognostic potentials of BCSC-related miRNAs in different subtypes (Luminal A and B, HER2 + and TNBC) of BC patients.
Expression analysis of 15 BCSC-related miRNAs was performed in 50 breast tumor tissues and 20 adjacent non-tumor tissues obtained from BC patients using the qRT-PCR method. The expression levels of miR-31 and miR-150-3p were significantly upregulated in the tumor tissues compared to the adjacent non-tumor tissues (p < 0.05). miR-31 expression upregulated in the Luminal A and Luminal B group compared to non-tumor tissue (p < 0.05). miR-31 expression was determined to be significantly higher in the Luminal group (Luminal A and B) compared to the aggressive group (HER2 + and TNBC) (p < 0.05). According to the ROC analysis, the area under the curve (AUC) of miR-31 and miR-150-3p were 0.66 with a sensitivity of 68% and a specificity of 70%. A significant inverse correlation was observed between miR-31 expression with metastatic carcinoma status, in situ component, and Ki67 value in tumors, and high miR-150-3p expression was correlated with p63 expression (p < 0.05).
miR-31 and miR-150-3p have the potential to serve as biomarkers for guiding diagnosis, evaluating prognosis, and metastatic process in patients with BC.
女性最常见的恶性肿瘤是乳腺癌（BC）。microRNAs（miRNAs）通过影响乳腺癌干细胞（BCSCs）在乳腺癌的发生和发展中发挥作用，但这些 miRNAs 对 BC 患者的诊断和预后作用仍不清楚。本研究旨在探讨不同亚型（Luminal A 和 B、HER2+和 TNBC）BC 患者中 BCSC 相关 miRNAs 的表达谱、诊断和预后潜力。
采用 qRT-PCR 法检测 50 例乳腺癌组织和 20 例癌旁非肿瘤组织中 15 种 BCSC 相关 miRNA 的表达。与癌旁非肿瘤组织相比，肿瘤组织中 miR-31 和 miR-150-3p 的表达水平显著上调（p<0.05）。miR-31 在 Luminal A 和 Luminal B 组中的表达高于非肿瘤组织（p<0.05）。miR-31 的表达在 Luminal 组（Luminal A 和 B）中明显高于侵袭性组（HER2+和 TNBC）（p<0.05）。根据 ROC 分析，miR-31 和 miR-150-3p 的曲线下面积（AUC）分别为 0.66，敏感性为 68%，特异性为 70%。miR-31 的表达与肿瘤转移癌状态、原位成分和 Ki67 值呈显著负相关，高 miR-150-3p 表达与 p63 表达相关（p<0.05）。
miR-31 和 miR-150-3p 有可能成为指导诊断、评估预后和转移性过程的生物标志物，用于 BC 患者。
