Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
College of Agronomy, Xinjiang Agricultural University, Urumqi, 830052, China.
Planta. 2024 Oct 1;260(5):110. doi: 10.1007/s00425-024-04524-w.
The GhEB1C gene of the EB1 protein family functions as microtubule end-binding protein and may be involved in the regulation of microtubule-related pathways to enhance resistance to Verticillium wilt. The expression of GhEB1C is induced by SA, also contributing to Verticillium wilt resistance. Cotton, as a crucial cash and oil crop, faces a significant threat from Verticillium wilt, a soil-borne disease induced by Verticillium dahliae, severely impacting cotton growth and development. Investigating genes associated with resistance to Verticillium wilt is paramount. We identified and performed a phylogenetic analysis on members of the EB1 family associated with Verticillium wilt in this work. GhEB1C was discovered by transcriptome screening and was studied for its function in cotton defense against V. dahliae. The RT-qPCR analysis revealed significant expression of the GhEB1C gene in cotton leaves. Subsequent localization analysis using transient expression demonstrated cytoplasmic localization of GhEB1C. VIGS experiments indicated that silencing of the GhEB1C gene significantly increased susceptibility of cotton to V. dahliae. Comparative RNA-seq analysis showed that GhEB1C silenced plants exhibited altered microtubule-associated protein pathways and flavonogen-associated pathways, suggesting a role for GhEB1C in defense mechanisms. Overexpression of tobacco resulted in enhanced resistance to V. dahliae as compared to wild-type plants. Furthermore, our investigation into the relationship between the GhEB1C gene and plant disease resistance hormones salicylic axid (SA) and jasmonic acid (JA) revealed the involvement of GhEB1C in the regulation of the SA pathway. In conclusion, our findings demonstrate that GhEB1C plays a crucial role in conferring immunity to cotton against Verticillium wilt, providing valuable insights for further research on plant adaptability to pathogen invasion.
GhEB1C 基因属于 EB1 蛋白家族，作为微管末端结合蛋白发挥功能，可能参与微管相关途径的调控，从而增强对黄萎病的抗性。GhEB1C 的表达受 SA 诱导，也有助于黄萎病抗性。棉花作为一种重要的经济作物和油料作物，面临着黄萎病的严重威胁，黄萎病是由大丽轮枝菌引起的土传病害，严重影响棉花的生长和发育。研究与黄萎病抗性相关的基因至关重要。本研究通过对与黄萎病相关的 EB1 家族成员进行鉴定和系统发育分析。通过转录组筛选发现 GhEB1C 基因，并研究其在棉花抵御大丽轮枝菌中的功能。RT-qPCR 分析显示 GhEB1C 基因在棉花叶片中表达量显著。随后利用瞬时表达进行定位分析显示 GhEB1C 定位于细胞质。VIGS 实验表明 GhEB1C 基因沉默显著增加了棉花对大丽轮枝菌的敏感性。比较 RNA-seq 分析表明 GhEB1C 沉默的植株中微管相关蛋白途径和类黄酮生物合成途径发生改变，表明 GhEB1C 参与了防御机制。与野生型植株相比，烟草中 GhEB1C 的过表达导致对大丽轮枝菌的抗性增强。此外，我们还研究了 GhEB1C 基因与植物抗病激素水杨酸（SA）和茉莉酸（JA）之间的关系，结果表明 GhEB1C 参与了 SA 途径的调控。综上所述，我们的研究结果表明 GhEB1C 在赋予棉花对黄萎病的免疫中起着关键作用，为进一步研究植物对病原体入侵的适应性提供了有价值的信息。
