Ortiz-Juza Maria M, Tormes-Vaquerano Jovan, Hegel Sophia M, Curtis Vincent R, Alghorazi Rizk A, Miller Noah W, McTaggart Ellora M, Pégard Nicolas C, Rodriguez-Romaguera Jose
Neuroscience Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.
Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Applied Physical Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.
STAR Protoc. 2024 Dec 20;5(4):103331. doi: 10.1016/j.xpro.2024.103331. Epub 2024 Sep 30.
A wide selection of behavioral assays in systems neuroscience relies on head-fixation protocols to integrate in vivo multi-photon imaging approaches. For this, simultaneous pupillometry and locomotion tracking in head-fixed mice are used to measure behavioral responses and identify neural correlates. Here, we present an open-source protocol for assembling a complete head-fixation system that integrates pupillometry and locomotion-estimated tracking with multi-photon calcium imaging. We include detailed procedures for head-fixation and for data collection.
系统神经科学中的多种行为分析方法依赖于头部固定方案来整合体内多光子成像方法。为此，在头部固定的小鼠中同时进行瞳孔测量和运动跟踪，以测量行为反应并识别神经关联。在这里，我们提出了一个开源方案，用于组装一个完整的头部固定系统，该系统将瞳孔测量和运动估计跟踪与多光子钙成像相结合。我们包括了头部固定和数据收集的详细程序。
