Gargani G, Pini G
Chemioterapia. 1985 Oct;4(5):406-12.
Authors emphasize the pathogenetic importance of fungous dimorphism and its consequence in the usefulness of antimycotic drugs. For the study of drugs' activity on C. albicans, two models for the evaluation on yeast or mycelial form were developed. The first model is of candida growth on an animal cell culture, where a good mycelial form develops during the first 24 hours: authors demonstrated a synergism of the amphotericin B-rifampin combination, which is less evident in the traditional in vitro tests, and some activity of tolcyclate. The second model is an intracutaneous injection in the guinea pig of C. albicans, which quickly develops in the mycelial form, infiltrating the skin layers. Some interesting differences were recorded, comparing amphotericin B and ketoconazole. Moreover the authors used a therapy test in mice, based mainly on examination of the kidney.
作者强调真菌双态性的发病机制重要性及其在抗真菌药物有效性方面的后果。为了研究药物对白色念珠菌的活性，开发了两种用于评估酵母或菌丝体形式的模型。第一个模型是念珠菌在动物细胞培养物上生长，在前24小时内会形成良好的菌丝体形式：作者证明了两性霉素B-利福平组合具有协同作用，这在传统的体外试验中不太明显，同时还证明了托西拉酯具有一定活性。第二个模型是将白色念珠菌皮内注射到豚鼠体内，其会迅速形成菌丝体形式并浸润皮肤层。比较两性霉素B和酮康唑时记录到了一些有趣的差异。此外，作者在小鼠身上进行了一项治疗试验，主要基于对肾脏的检查。
