Key Laboratory of Research on Clinical Molecular Diagnosis for High Incidence Diseases in Western Guangxi of Guangxi Higher Education Institutions, Reproductive Medicine of Guangxi Medical and Health Key Discipline Construction Project, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise 533000, China; Industrial College of Biomedicine and Health Industry, Youjiang Medical University for Nationalities, Baise 533000, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Ecotoxicol Environ Saf. 2024 Oct 15;285:117137. doi: 10.1016/j.ecoenv.2024.117137. Epub 2024 Sep 30.
Deoxynivalenol (DON) is a secondary metabolite of Fusarium fungi and belonged to trichothecenes, and it widely presents in various food commodities. Previous studies have highlighted its potent toxicity, adversely affecting the growth, development, and reproductive in both humans and animals. However, the potential impact of DON on porcine oocyte organelles remains elusive. In present study, we delved into the toxic effects of DON on mitochondria, endoplasmic reticulum, Golgi during the porcine oocyte maturation. Our findings revealed that DON exposure significantly impeded granulosa cell diffusion and the expulsion of the first polar body. Additionally, mitochondrial fluorescence intensity and membrane potential underwent notable alterations under DON exposure. Notably, lysosomal fluorescence intensity decreased significantly, suggesting protein degradation and potential autophagy, which was further corroborated by the enhanced fluorescence intensity of LC3. Furthermore, endoplasmic reticulum fluorescence intensity declined, and DON exposure elevated endoplasmic reticulum stress levels, evident from the upregulated expression of GRP78. Concurrently, we observed disruption in the fusiform cortex distribution of the Golgi apparatus, characterized by reduced Golgi apparatus fluorescence intensity and GM130 expression. Collectively, our results indicate that DON exposure profoundly affects the fundamental functions of porcine oocyte organelles during meiosis and maturation.
脱氧雪腐镰刀菌烯醇（DON）是镰刀菌真菌的次生代谢物，属于单端孢霉烯族化合物，广泛存在于各种食品中。先前的研究强调了其强烈的毒性，对人类和动物的生长、发育和生殖都有不良影响。然而，DON 对猪卵母细胞细胞器的潜在影响仍不清楚。在本研究中，我们研究了 DON 在猪卵母细胞成熟过程中对线粒体、内质网和高尔基体的毒性作用。我们的研究结果表明，DON 暴露显著阻碍了颗粒细胞的扩散和第一极体的排出。此外，线粒体荧光强度和膜电位在 DON 暴露下发生显著变化。值得注意的是，溶酶体荧光强度显著降低，提示蛋白质降解和潜在的自噬，这进一步得到 LC3 荧光强度增强的证实。此外，内质网荧光强度下降，DON 暴露导致内质网应激水平升高，GRP78 的表达上调。同时，我们观察到高尔基体的梭形皮质分布中断，表现为高尔基体荧光强度和 GM130 表达降低。总之，我们的研究结果表明，DON 暴露在减数分裂和成熟过程中对猪卵母细胞细胞器的基本功能有深远影响。
