白芷通过促进成骨细胞分化减轻模拟微重力诱导的骨质流失。

Angelicae dahuricae radix alleviates simulated microgravity induced bone loss by promoting osteoblast differentiation.

作者信息

Liang Xuechao, Jiang Shanfeng, Su Peihong, Yin Chong, Jiang Wei, Gao Junhong, Liu Zhiyong, Li Yuhang, Wang Weisi, Qian Airong, Tian Ye

机构信息

Lab for Bone Metabolism, Key Lab for Space Biosciences and Biotechnology, Xi'an Key Laboratory of Special Medicine and Health Engineering, Research Center for Special Medicine and Health Systems Engineering, NPU-UAB Joint Laboratory for Bone Metabolism, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China.

Shaanxi Provincial Key Laboratory of Ischemic Cardiovascular Disease, Institute of Basic and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi, 710021, China.

出版信息

NPJ Microgravity. 2024 Oct 1;10(1):91. doi: 10.1038/s41526-024-00433-0.

DOI:10.1038/s41526-024-00433-0

PMID:39353918

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11445575/

Abstract

Bone loss caused by long-duration spaceflight seriously affects the skeletal health of astronauts. There are many shortcomings in currently available treatments for weightlessness-induced bone loss. The aim of this study was to evaluate the preventive effect of Angelica dahuricae Radix (AR) on simulated microgravity-induced bone loss. Here, we established a hind limb unloading (HLU) mouse model and treated HLU mice with AR (2 g/kg) for 4 weeks. Results indicated that AR significantly inhibited simulated microgravity-induced bone loss. In addition, the components in AR were analyzed using UPLC-MS/MS; results showed that a total of 224 compounds were detected in AR, which mainly contained 7 classes of components. Moreover, the network pharmacological predictions suggested that active ingredients of AR might act on PTGS2 to prevent bone loss. These results elucidate the efficacy of AR in preventing microgravity-induced bone loss and its potential for use in protecting the bone health of astronauts.

摘要

长期太空飞行导致的骨质流失严重影响宇航员的骨骼健康。目前针对失重诱导骨质流失的治疗方法存在诸多不足。本研究旨在评估白芷（AR）对模拟微重力诱导骨质流失的预防作用。在此，我们建立了后肢卸载（HLU）小鼠模型，并用AR（2 g/kg）对HLU小鼠进行了4周的治疗。结果表明，AR显著抑制了模拟微重力诱导的骨质流失。此外，采用超高效液相色谱-串联质谱法（UPLC-MS/MS）分析了AR中的成分；结果显示，在AR中总共检测到224种化合物，主要包含7类成分。此外，网络药理学预测表明，AR的活性成分可能作用于PTGS2以预防骨质流失。这些结果阐明了AR在预防微重力诱导骨质流失方面的功效及其在保护宇航员骨骼健康方面的应用潜力。