KM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of Korea.
Korean Convergence Medicine Major KIOM, University of Science & Technology (UST), 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 34054, Republic of Korea.
Int J Mol Sci. 2023 Sep 28;24(19):14715. doi: 10.3390/ijms241914715.
radix has a long history of traditional use in China and Korea for treating headaches, cold-damp pain and skin diseases. Despite various pharmacological studies on , its impact on bones remains unclear. Hence, this study investigated the inhibitory effect of 's radix water extract (WEAD) on osteoclast differentiation. In vitro experiments showed that WEAD effectively suppresses osteoclast differentiation. Treatment of an osteoclast precursor with WEAD significantly suppressed the expression of nuclear factor of activated T-cells 1 (NFATc1), essential transcription factor for osteoclastogenesis, while increasing the expression of negative regulators, interferon regulatory factor 8 (Irf8) and v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (MafB). Consistent with the in vitro findings, the oral administration of WEAD (100 and 300 mg/kg/day) to mice subjected to surgical ovariectomy for a duration of six weeks alleviated bone loss, while also mitigating weight gain and liver fat accumulation. In addition, we also identified phytochemicals present in WEAD, known to regulate osteoclastogenesis and/or bone loss. These results suggest the potential use of WEAD for treating various bone disorders caused by excessive bone resorption.
该植物在中、韩两国具有悠久的传统医学应用历史，可用于治疗头痛、寒湿疼痛和皮肤病。尽管已经有大量关于 的药理学研究，但它对骨骼的影响仍不清楚。因此，本研究调查了 的根茎水提物（WEAD）对破骨细胞分化的抑制作用。体外实验表明，WEAD 能有效抑制破骨细胞分化。用 WEAD 处理破骨细胞前体，能显著抑制破骨细胞生成的关键转录因子核因子活化 T 细胞 1（NFATc1）的表达，同时增加负调控因子干扰素调节因子 8（Irf8）和 v-maf 肌肉腱膜纤维肉瘤癌基因同源物 B（MafB）的表达。与体外研究结果一致，WEAD（100 和 300 mg/kg/天）灌胃给药 6 周的去卵巢小鼠，能缓解骨丢失，同时减轻体重增加和肝脏脂肪堆积。此外，我们还鉴定出 WEAD 中存在的调节破骨细胞生成和/或骨丢失的植物化学物质。这些结果表明，WEAD 可能具有治疗由过度骨吸收引起的各种骨骼疾病的潜力。
