尿液中前列腺素代谢产物的 LC-MS/MS 分析。
Prostaglandin Metabolites Analysis in Urine by LC-MS/MS.
机构信息
PharmaCadence Analytical Services, LLC, Hatfield, PA, USA.
Immunology and Respiratory Disease, Boehringer-Ingelheim Pharmaceuticals Inc., Ridgefield, CT, USA.
出版信息
Methods Mol Biol. 2025;2855:147-154. doi: 10.1007/978-1-0716-4116-3_9.
The analysis of prostaglandin urinary metabolites is valuable for assessing physiological processes and identifying disease biomarkers. These metabolites, derived from the breakdown of prostaglandins, offer a noninvasive means to gauge prostaglandin production and its potential impact on various biological functions. We report an efficient LC-MS method of four commonly analyzed prostaglandin urinary metabolites including tetranor-PGEM (derived from PGE), tetranor-PGDM, 11β-PGF, and 2,3-dinor-11β-PGF (derived from PGD). Each metabolite possesses distinct characteristics and clinical applications, collectively contributing to our understanding of prostaglandin-mediated pathways.
分析前列腺素尿代谢产物对于评估生理过程和识别疾病生物标志物具有重要价值。这些代谢产物来源于前列腺素的分解，提供了一种非侵入性的方法来衡量前列腺素的产生及其对各种生物功能的潜在影响。我们报告了一种高效的 LC-MS 方法，用于分析四种常见的前列腺素尿代谢产物，包括四氢前列腺素 E 甲酯（源自 PGE）、四氢前列腺素 DM、11β-PGF 和 2,3-二去甲-11β-PGF（源自 PGD）。每种代谢产物都具有独特的特征和临床应用，共同有助于我们理解前列腺素介导的途径。
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