Tang Ling, Sun Chenyun, He Yuxia, Hou Haiyu, Shang Yaqi, Li Li, Mao Yuling, Li Lei
Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
Key Laboratory for Reproductive Medicine of Guangdong Province, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
BMC Pregnancy Childbirth. 2024 Oct 1;24(1):629. doi: 10.1186/s12884-024-06825-0.
Explore the effect of blastomere cell number on ART outcome of fresh embryo transfer on day 3.
Retrospective analysis of 540 fresh single day 3 embryo transfer cycles at the Reproductive Center of the Third Affiliated Hospital of Guangzhou Medical University from January 1, 2018 to October 31, 2022. Patients were divided into 5-6 cell group (n = 55), 7-9 cell group (n = 457), and ≥ 10 cell group(n = 28) based on the number of blastomeres. Single factor analysis of variance and Pearson's chi square test were used to compare the basic data, cycle information, pregnancy outcome and neonatal outcome. Univariate logistic regression was used to correct for confounding factors and analyze the influencing factors of pregnancy outcome.
The positive HCG rate were 20%, 43%, 25% for the 5-6-cell, 7-9 cell and ≥ 10 cell groups respectively, with statistically significant differences (P < 0.001). The clinical pregnancy rate was 18%, 42%,21%, respectively (P < 0.001). The live birth rates were 13%, 34%,21% with P-value less than 0.05 which is statistically significant. In order to exclude the influence of confounding factors, multivariable logistic regression analysis was performed, and the outcomes were consistent with previous findings. There were no significant differences found in neonatal outcome between groups (P > 0.05).
The results suggested that intermediate cleaving embryos (7-9 cell) still presents the highest clinical potential. Fast and slow cleaving embryos are not conducive to the ART outcome.
探讨卵裂球细胞数量对第3天新鲜胚胎移植辅助生殖技术结局的影响。
回顾性分析2018年1月1日至2022年10月31日在广州医科大学附属第三医院生殖中心进行的540个新鲜第3天单胚胎移植周期。根据卵裂球数量将患者分为5 - 6细胞组（n = 55）、7 - 9细胞组（n = 457）和≥10细胞组（n = 28）。采用单因素方差分析和Pearson卡方检验比较基础数据、周期信息、妊娠结局和新生儿结局。采用单因素logistic回归校正混杂因素并分析妊娠结局的影响因素。
5 - 6细胞组、7 - 9细胞组和≥10细胞组的HCG阳性率分别为20%、43%、25%，差异有统计学意义（P < 0.001）。临床妊娠率分别为18%、42%、21%（P < 0.001）。活产率分别为13%、34%、21%，P值小于0.05，差异有统计学意义。为排除混杂因素的影响，进行多变量logistic回归分析，结果与之前的发现一致。各组间新生儿结局差异无统计学意义（P > 0.05）。
结果表明，中等分裂胚胎（7 - 9细胞）仍具有最高的临床潜能。分裂过快和过慢的胚胎不利于辅助生殖技术结局。
