Aydın Bilge, Alkuyruk Satuk Buğra, Tekman Enes, Yuca Hafize, Karadayı Mehmet, Gülşahin Yusuf, Çeçen Ömer, Bona Gülnur Ekşi, Demirci Betül, Bona Mehmet, Karakaya Songül
Department of Pharmacognosy, Erzincan Binali Yıldırım University, Erzincan, Türkiye.
Department of Pharmaceutical Microbiology, Ankara University, Ankara, Türkiye.
Protoplasma. 2025 Mar;262(2):279-298. doi: 10.1007/s00709-024-01992-5. Epub 2024 Oct 2.
Cyclotrichium origanifolium, a plant widely used in Eastern and Southern Anatolia for culinary purposes, was subject of this study, which aimed to comprehensively evaluate its potential therapeutic applications. This research stands out due to its holistic approach, combining morpho-anatomical studies, chemical, and biological analyses to explore antioxidant, antidiabetic, anticholinesterase, genotoxic, and anti-genotoxic effects of methanolic and aqueous extracts, as well as flowering aerial part essential oil. It is a perennial plant, typically ranging from 10 to 40 cm in height, with a suffrutescent and highly branched growth habit. Essential oils are produced within glandular trichomes. Oil, analyzed via GC-MS/MS, revealed 24 compounds accounting for 96.4% of oil, with isomenthone (52.4%), pulegone (23.4%), and β-pinene (9.5%) as predominant components. These findings are significant as they provide new insights into chemical composition of oils, particularly highlighting pharmacologically active compounds. Methanol extract exhibited superior antioxidant activity, correlated with high phenol and tannin content. Essential oil showed moderate inhibition of α-amylase (49.54%) and mild inhibition of acetylcholinesterase (11.84%) and butyrylcholinesterase (16.93%), suggesting potential in managing oxidative stress and neurodegenerative diseases. Study also conducted biosafety evaluations using Ames/Salmonella and Allium tests, essential for assessing genotoxic and antigenotoxic potential of natural products. Notably, significant antimicrobial effects were identified, particularly against Pseudomonas aeruginosa and Enterococcus faecalis. Comprehensive analysis and discovery of significant bioactivities position this research as a valuable contribution to field, distinguishing it from previous studies on similar species. This study provides a foundational understanding of morpho-anatomical, pharmacological, biological properties of plant, opening avenues for future research.
牛至叶环香堇是一种在安纳托利亚东部和南部广泛用于烹饪的植物，是本研究的对象，该研究旨在全面评估其潜在的治疗应用。这项研究因其整体方法而脱颖而出，它结合了形态解剖学研究、化学和生物学分析，以探索甲醇提取物、水提取物以及开花地上部分精油的抗氧化、抗糖尿病、抗胆碱酯酶、遗传毒性和抗遗传毒性作用。它是一种多年生植物，高度通常在10至40厘米之间，具有半灌木状且高度分枝的生长习性。精油在腺毛中产生。通过气相色谱 - 质谱联用/质谱分析的油显示有24种化合物，占油的96.4%，其中异薄荷酮（52.4%）、长叶薄荷酮（23.4%）和β-蒎烯（9.5%）为主要成分。这些发现具有重要意义，因为它们为油的化学成分提供了新的见解，特别突出了具有药理活性的化合物。甲醇提取物表现出卓越的抗氧化活性，这与高酚和单宁含量相关。精油对α-淀粉酶有中度抑制作用（49.54%），对乙酰胆碱酯酶有轻度抑制作用（11.84%），对丁酰胆碱酯酶有轻度抑制作用（16.93%），表明其在应对氧化应激和神经退行性疾病方面具有潜力。该研究还使用艾姆斯/沙门氏菌试验和葱属试验进行了生物安全性评估，这对于评估天然产物的遗传毒性和抗遗传毒性潜力至关重要。值得注意的是，发现了显著的抗菌作用，特别是对铜绿假单胞菌和粪肠球菌。全面的分析和重要生物活性的发现使这项研究成为该领域的一项有价值的贡献，使其有别于以往对类似物种的研究。这项研究为该植物的形态解剖学、药理学、生物学特性提供了基础认识，为未来的研究开辟了道路。
