Guzel Abdussamat, Aksit Huseyin, Elmastas Mahfuz, Erenler Ramazan
Department of Chemistry, Faculty of Science and Art, Gaziosmanpasa University, Tokat, Turkey.
Pharmacogn Mag. 2017 Apr-Jun;13(50):316-320. doi: 10.4103/0973-1296.204556. Epub 2017 Apr 18.
Medicinal and aromatic plants play a significant role in drug discovery and development process. Flavonoids, revealing a wide spectrum of biological activities, extensively found in plants are important secondary metabolites.
Aerial parts of were collected, dried, and boiled in water then extracted with hexane, ethyl acetate, and -butanol. Total phenolic content, DPPH scavenging activity, reducing power (FRAP) activity, and ABTS scavenging activity assays were applied for all extracts. The ethyl acetate extract revealing the most antioxidant activity as well as including the highest phenolic contents was subjected to chromatographic techniques (column chromatography, sephadex LH-20, semipreparative HPLC) to isolate the active compounds. The structure of isolated compounds were elucidated by spectroscopic methods (1D NMR, 2D NMR, and LC-TOF/MS).
Isosakuranetin (1), eriodictyol (2), luteolin (3), naringenin (4), and apigenin (5) were isolated and identified. All isolated flavonoids displayed the excellent antioxidant activity.
The isolated flavonoids and also plant extract have potency to be a natural antioxidant.
Five flavonoids were isolated from Isolated compounds revealed the good antioxidant activity has a potency to be used in food industries DPPH•: 1,1-diphenyl-2-picryl-hydrazyl free radical, ABTS•+: 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), UV:Ultraviolet, DNA: Deoxyribonucleic acid, BHT: Butylated hydroxytoluene, BHA: Butylated hydroxyanisole, HPLC: High performance liquid chromatography.
药用和芳香植物在药物发现与开发过程中发挥着重要作用。黄酮类化合物是植物中广泛存在的重要次生代谢产物，具有广泛的生物活性。
采集[植物名称]的地上部分，干燥后用水煮沸，然后用己烷、乙酸乙酯和正丁醇萃取。对所有提取物进行总酚含量、DPPH清除活性、还原力（FRAP）活性和ABTS清除活性测定。乙酸乙酯提取物显示出最强的抗氧化活性且酚含量最高，对其采用色谱技术（柱色谱、葡聚糖LH - 20、半制备高效液相色谱）分离活性化合物。通过光谱方法（一维核磁共振、二维核磁共振和液相色谱 - 飞行时间质谱）阐明分离出的化合物结构。
分离并鉴定出异樱花素（1）、圣草酚（2）、木犀草素（3）、柚皮素（4）和芹菜素（5）。所有分离出的黄酮类化合物均表现出优异的抗氧化活性。
分离出的黄酮类化合物以及植物提取物有潜力成为天然抗氧化剂。
从[植物名称]中分离出五种黄酮类化合物。分离出的化合物显示出良好的抗氧化活性，有潜力用于食品工业。DPPH•：1,1 - 二苯基 - 2 - 苦基肼自由基，ABTS• +：2,2'-联氮 - 双 -（3 - 乙基苯并噻唑啉 - 6 - 磺酸），UV：紫外线，DNA：脱氧核糖核酸，BHT：丁基羟基甲苯，BHA：丁基羟基茴香醚，HPLC：高效液相色谱。
