Brundin P, Barbin G, Isacson O, Mallat M, Chamak B, Prochiantz A, Gage F H, Björklund A
Neurosci Lett. 1985 Oct 24;61(1-2):79-84. doi: 10.1016/0304-3940(85)90404-5.
Fetal rat dopamine (DA) neurons were cultured in vitro for a 6-day period and transported, after redissociation, for 2 days prior to being grafted to the neostriatum of adult rats with 6-hydroxydopamine lesions of the ascending nigrostriatal pathway. In 2 of the 5 graft recipients that were tested for amphetamine-induced motor asymmetry, the grafts eliminated the lesion-induced turning behaviour within 3-6 weeks after transplantation. Fluorescence histochemistry revealed surviving grafts in all 6 recipients at 7 weeks after transplantation, containing between 42 and 125 DA neurons. The number of surviving DA neurons in the 3 non-compensated rats was below the minimum number of cells previously found to be necessary for functional effects on turning behaviour to occur.
将胎鼠多巴胺（DA）神经元在体外培养6天，再解离后运输2天，然后移植到黑质纹状体上行通路经6-羟基多巴胺损伤的成年大鼠的新纹状体中。在5只接受移植并测试安非他明诱导的运动不对称性的大鼠中，有2只的移植物在移植后3 - 6周内消除了损伤诱导的旋转行为。荧光组织化学显示，移植后7周时，所有6只接受移植的大鼠体内都有存活的移植物，其中含有42至125个DA神经元。3只未出现补偿效应的大鼠中存活的DA神经元数量低于先前发现的对旋转行为产生功能影响所需的最小细胞数量。
