Chen Le, Ai Yuying, Wu Ruonan, Guo Zhaoyan, Li Yang, Li Jie, Qu Feng, Duan Shun, Xu Fu-Jian
State Key Laboratory of Chemical Resource Engineering, Key Lab of Biomedical Materials of Natural Macromolecules ( Beijing University of Chemical Technology), Ministry of Education, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
Beijing Research Institute of Chemical Industry, Sinopec, Beijing 100013, China.
BME Front. 2024 Oct 2;5:0066. doi: 10.34133/bmef.0066. eCollection 2024.
We aim to develop a dual-functional bone regeneration scaffold (Q-D) with antibacterial and osteogenic properties for infected bone defect treatment. This study provides insights into antibacterial components that could be combined with naturally derived materials through a facile Schiff base reaction, offering a potential strategy to enhance antibacterial properties. Naturally derived decalcified bone matrix (DBM) has been reported to be porous and biodegradable. DBM can induce various cell differentiations and participate in immune regulation, making it an ideal bone regeneration scaffold for bone defects. However, DBM does not exhibit antimicrobial properties. Therefore, it is essential to develop antibacterial functionalization method for DBM. DBM was modified with a macromolecular quaternary ammonium salt (QPEI). A series of Q-D with tunable feeding ratios were synthesized through Schiff base reaction. The morphology, chemical property, in vitro antibacterial efficiency, in vitro biocompatibility, osteogenic property, and in vivo anti-infection performances were characterized. All Q-D exhibited marked antibacterial properties. Small adjustments in feed concentration could not induce changes in antibacterial properties. However, cell viability slightly decreased with increasing feed concentration. Q10-D demonstrated significant antibacterial properties and could promote recovery of infected bone defect in an animal model. Q-D shows marked antibacterial properties and good biocompatibility. Moreover, Q10-D could be a potential choice for infected bone defects.
我们旨在开发一种具有抗菌和成骨特性的双功能骨再生支架（Q-D），用于治疗感染性骨缺损。本研究深入探讨了可通过简便的席夫碱反应与天然衍生材料结合的抗菌成分，为增强抗菌性能提供了一种潜在策略。据报道，天然衍生的脱钙骨基质（DBM）具有多孔性和可生物降解性。DBM可诱导多种细胞分化并参与免疫调节，使其成为治疗骨缺损的理想骨再生支架。然而，DBM不具有抗菌性能。因此，开发DBM的抗菌功能化方法至关重要。用大分子季铵盐（QPEI）对DBM进行修饰。通过席夫碱反应合成了一系列投料比可调的Q-D。对其形态、化学性质、体外抗菌效率、体外生物相容性、成骨性能和体内抗感染性能进行了表征。所有Q-D均表现出显著的抗菌性能。进料浓度的微小调整不会引起抗菌性能的变化。然而，细胞活力随进料浓度的增加而略有下降。Q10-D表现出显著的抗菌性能，并能促进动物模型中感染性骨缺损的恢复。Q-D表现出显著的抗菌性能和良好的生物相容性。此外，Q10-D可能是治疗感染性骨缺损的一个潜在选择。
