Department of Stomatology, Affiliated Hospital of Yunnan University, Kunming, Yunnan 650000, PR China.
J Med Microbiol. 2024 Oct;73(10). doi: 10.1099/jmm.0.001897.
Peri-implantitis is a plaque-associated disease that leads to implant loss and arises from bacterial biofilms on the surface of the implant. Smoking is a risk factor for peri-implantitis and impedes treatment effectiveness. Additionally, aryl hydrocarbon receptor (AHR), IL-6, and IL-22 levels are related to peri-implantitis. We aimed to investigate the effects of nicotine on inflammatory response, bacterial growth and biofilm formation. We hypothesized that nicotine promoted pathogenic bacterial growth and biofilm formation, thereby aggravating inflammation. The expression of AHR, IL-6 and IL-22 was measured in peri-implant sulci fluid using quantitative PCR and Western blot analyses. The cementum was incubated with bacterial suspension including , and and treated with 100, 200, 250 and 300 µg ml nicotine, and then, the absorbance and number of colony-forming units were detected. Biofilm formation was evaluated using the tissue culture plate method and safranin O staining. Carbohydrates and proteins were measured by the phenol-sulfuric acid method and the bicinchoninic acid method, respectively. The results indicated that smoking increased the levels of AHR, IL-6 and IL-22. Functionally, nicotine promoted the growth of , and . Additionally, it promoted the biofilm formation of these bacteria and increased the contents of carbohydrates and proteins. Nicotine promoted bacterial growth and biofilm build-up, suggesting that smoking may aggravate the progression of peri-implantitis.
种植体周围炎是一种与菌斑相关的疾病，会导致种植体丧失，其病因是种植体表面的细菌生物膜。吸烟是种植体周围炎的一个风险因素，并会影响治疗效果。此外，芳香烃受体 (AHR)、白细胞介素 6 (IL-6) 和白细胞介素 22 (IL-22) 的水平与种植体周围炎有关。我们旨在研究尼古丁对炎症反应、细菌生长和生物膜形成的影响。我们假设尼古丁促进了致病菌的生长和生物膜形成，从而加重了炎症。使用定量聚合酶链反应和 Western blot 分析测量种植体周围沟液中的 AHR、IL-6 和 IL-22 的表达。将牙骨质与包含 、 和 的细菌悬浮液孵育，并分别用 100、200、250 和 300μg/ml 的尼古丁处理，然后检测吸光度和菌落形成单位数。使用组织培养板法和 safranin O 染色评估生物膜形成。通过苯酚-硫酸法和双缩脲法分别测量碳水化合物和蛋白质。结果表明，吸烟增加了 AHR、IL-6 和 IL-22 的水平。功能上，尼古丁促进了 、 和 的生长。此外，它促进了这些细菌的生物膜形成，并增加了碳水化合物和蛋白质的含量。尼古丁促进了细菌的生长和生物膜的形成，表明吸烟可能会加重种植体周围炎的进展。
