Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, Indianapolis, Indiana, USA.
Department of Oral Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.
J Periodontol. 2021 Aug;92(8):1151-1162. doi: 10.1002/JPER.20-0333. Epub 2020 Dec 10.
Removal of dental plaque and local application of local chemical adjuncts, such as chlorhexidine (CHX), have been used to control and treat peri-implant disease. However, these methods can damage the surface properties of the implants or promote bacterial resistance. The application of ozone as an adjunctive treatment represents a new approach in the management of peri-implantitis. Thus, the purpose of this study was to evaluate the antimicrobial effect of ozonized physiological saline solution in different concentrations against oral biofilms developed on titanium surface.
Single and multi-species biofilms of Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus oralis were formed on titanium specimens for 5 days in anaerobic conditions. Biofilms were treated with ozonized saline solution at different concentrations (25, 50, and 80 μg/NmL), for 30 seconds and 1 minute. CHX (0.12%) and saline solution (0.89% NaCl) were used as positive and negative controls, respectively. Bacterial viability was quantified by colony forming units (CFU mL ), and biofilm images were acquired by confocal laser scanning microscopy (CLSM). Data were analyzed by parametric test (ANOVA) with Tukey post-hoc test (P < 0.05).
Ozonized saline solution showed antibiofilm activity at a concentration of 80 μg/NmL for 30 seconds and 1 minute, reducing, mainly, Porphyromonas gingivalis viability, with 2.78 and 1.7 log CFU mL of reduction in both single and multi-species biofilms, respectively, when compared to the control (saline), whereas CHX reduced 1.4 and 1.2 log CFU mL .
Ozonized saline solution has antibiofilm activity, with better effect when applied for 1 minute at 80 μg/NmL, being a promising candidate therapy for the treatment of peri-implant diseases.
去除牙菌斑和局部应用局部化学助剂，如洗必泰（CHX），已被用于控制和治疗种植体周围疾病。然而，这些方法可能会损害种植体的表面性能或促进细菌耐药性。臭氧作为辅助治疗的应用代表了一种治疗种植体周围炎的新方法。因此，本研究旨在评估不同浓度臭氧生理盐水溶液对钛表面形成的口腔生物膜的抗菌效果。
在厌氧条件下，将牙龈卟啉单胞菌、核梭杆菌和口腔链球菌的单种和多种生物膜在钛标本上形成 5 天。用不同浓度（25、50 和 80μg/NmL）的臭氧生理盐水溶液处理生物膜 30 秒和 1 分钟。CHX（0.12%）和生理盐水（0.89%NaCl）分别作为阳性和阴性对照。通过平板计数法（CFU/mL）量化细菌活力，并通过共聚焦激光扫描显微镜（CLSM）获取生物膜图像。数据采用参数检验（ANOVA）和 Tukey 事后检验（P<0.05）进行分析。
臭氧生理盐水溶液在 80μg/NmL 浓度下作用 30 秒和 1 分钟具有抗生物膜活性，主要降低牙龈卟啉单胞菌的活力，在单种和多种生物膜中分别减少了 2.78 和 1.7 log CFU/mL，与对照组（生理盐水）相比，而 CHX 减少了 1.4 和 1.2 log CFU/mL。
臭氧生理盐水溶液具有抗生物膜活性，在 80μg/NmL 浓度下作用 1 分钟效果更好，是治疗种植体周围疾病的有前途的候选治疗方法。
