Ciaffoni S, Campo G
Quad Sclavo Diagn. 1985 Jun;21(2):225-35.
The authors examined 46 patients with autoimmune thrombocytopenic Purpura and 17 patients with autoimmune neutropenia, with the direct and indirect immunofluorescence method. They intended to verify the sensibility of such methods and to determine the class of immunoglobulins. In the PTA the direct PSIFT was positive in 26 of the 46 examined patients, while the indirect in 15 cases. In the GPA the direct GIFT was positive in 9 of the 17 patients and the indirect in 6 cases. By using FITC-labelled monospecific anti-immunoglobulin reagents, in the PTA we have found IgG in the 57.7% of the patients, IgM in the 11.5% and IgG + IgM in 30.8%; in the GPA we have found IgG in the 66% of the cases, IgM in 11% and IgG + IgM in 23%. These methods have shown a good reliability and sensibility, but also delicacy.
作者采用直接和间接免疫荧光法检查了46例自身免疫性血小板减少性紫癜患者和17例自身免疫性中性粒细胞减少症患者。他们旨在验证这些方法的敏感性并确定免疫球蛋白类别。在血小板减少性紫癜中，46例受检患者中有26例直接血小板免疫荧光试验（PSIFT）呈阳性，间接法有15例呈阳性。在粒细胞减少性紫癜中，17例患者中有9例直接粒细胞免疫荧光试验（GIFT）呈阳性，间接法有6例呈阳性。通过使用异硫氰酸荧光素（FITC）标记的单特异性抗免疫球蛋白试剂，在血小板减少性紫癜中，我们发现57.7%的患者有IgG，11.5%有IgM，30.8%有IgG + IgM；在粒细胞减少性紫癜中，66%的病例有IgG，11%有IgM，23%有IgG + IgM。这些方法显示出良好的可靠性、敏感性，也有一定的精细度。
