Lei Yu, Ji Zhiyong, Xiang Wei, Duan Liming
Department of Emergency Medicine, Lequn Branch, The First Hospital of Jilin University, Changchun 130021, China.
Department of Emergency Medicine, Lequn Branch, The First Hospital of Jilin University, Changchun 130021, China.
Bioorg Chem. 2024 Dec;153:107856. doi: 10.1016/j.bioorg.2024.107856. Epub 2024 Sep 30.
Organic luminogens with dual-state emission (DSE) have garnered widespread attention due to their versatility in the forms of both dilute solutions and solids. Despite the growing interest, most research on DSE focuses primarily on molecule design and photophysical investigation, with limited exploration of their practical applications. In this study, we introduce a novel fluorescent molecule, PCT, featuring a distinct D-π(A)-D' electronic structure. PCT exhibited efficient DSE properties, with high quantum yields in both dilute solutions (Φ = 52.3 %) and solid-state (Φ = 74.6 %). Taking advantage of PCT's lipophilicity, we demonstrated its potential for targeted lipid droplet (LD) imaging in living cells and its utility in monitoring LD depletion during cellular starvation. To further enhance its applicability in photodynamic therapy (PDT), PCT was encapsulated within the amphiphilic triblock copolymer Pluronic F127, forming PCT@F127 nanoparticles with improved colloidal stability. These nanoparticles efficiently generated singlet oxygen (O) under white light irradiation, achieving a O quantum yield of 57.2 %. In vitro studies on MCF-7 cells revealed significant O generation and potent phototoxicity, leading to marked cell apoptosis and necrosis. These results underscore PCT's multifunctionality as a DSEgen, with promising applications in both bioimaging and PDT.
具有双态发射（DSE）的有机发光体因其在稀溶液和固体形式中的多功能性而受到广泛关注。尽管兴趣日益浓厚，但大多数关于DSE的研究主要集中在分子设计和光物理研究上，对其实际应用的探索有限。在本研究中，我们引入了一种新型荧光分子PCT，其具有独特的D-π（A）-D'电子结构。PCT表现出高效的DSE特性，在稀溶液（Φ = 52.3%）和固态（Φ = 74.6%）中均具有高量子产率。利用PCT的亲脂性，我们展示了其在活细胞中靶向脂质滴（LD）成像的潜力及其在监测细胞饥饿期间LD消耗中的效用。为了进一步提高其在光动力疗法（PDT）中的适用性，PCT被封装在两亲性三嵌段共聚物Pluronic F127中，形成具有改善的胶体稳定性的PCT@F127纳米颗粒。这些纳米颗粒在白光照射下有效地产生单线态氧（O），实现了57.2%的O量子产率。对MCF-7细胞的体外研究显示出显著的O产生和强大的光毒性，导致明显的细胞凋亡和坏死。这些结果强调了PCT作为一种DSEgen的多功能性，在生物成像和PDT中均具有广阔的应用前景。
