Browne M J, Dodd I, Carey J E, Chapman C G, Robinson J H
Thromb Haemost. 1985 Aug 30;54(2):422-4.
Extra copies of the human tissue-type plasminogen activator (t-PA) gene were introduced into the Bowes melanoma cell line. We obtained a recombinant cell line (TRBM6) which secretes approximately ten-fold more t-PA than the parent cell line. The identity of the plasminogen activator made by the new cell line was confirmed by sizing on sodium dodecyl sulphate polyacrylamide gels and by specific quenching using anti-t-PA antibody. We estimate that the recombinant line produces t-PA at a rate of approximately 3 pg/cell/24 hr and that t-PA accumulates in the harvest medium at a rate of approximately 4000 International t-PA Units/ml/24 hr.
将人组织型纤溶酶原激活剂（t-PA）基因的额外拷贝导入鲍伊斯黑色素瘤细胞系。我们获得了一个重组细胞系（TRBM6），其分泌的t-PA比亲本细胞系多大约十倍。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定大小以及使用抗t-PA抗体进行特异性淬灭，证实了新细胞系产生的纤溶酶原激活剂的特性。我们估计重组细胞系产生t-PA的速率约为3 pg/细胞/24小时，并且t-PA在收获培养基中的积累速率约为4000国际单位t-PA/毫升/24小时。
