Almajidi Yasir Qasim, Muslim Rana Kadum, Issa Anmar A, Al-Musawi Mastafa H, Shahriari-Khalaji Mina, Mirhaj Marjan
Department of Pharmaceutics, College of Pharmacy, Al-Nahrain university, Baghdad, Iraq.
Department of Pharmacy, Baghdad College of Medical Sciences, Baghdad, Iraq.
Int J Biol Macromol. 2024 Nov;280(Pt 4):136214. doi: 10.1016/j.ijbiomac.2024.136214. Epub 2024 Oct 1.
Despite substantial advancements in wound dressing development, effective skin repair remains a significant challenge, largely due to the persistent issue of recurrent infections. Three-dimensional printed constructs that integrate bioactive and antibacterial agents hold significant potential to address this challenge. In this study, a 3D-printed hydrogel scaffold composed of polyallylamine hydrochloride (PAH) and pectin (Pc), incorporated with mupirocin (Mp)-loaded quaternized chitosan nanoparticles (QC NPs) was fabricated. The primary objective of this study was to facilitate a controlled and sustained release of Mp via the QC NPs. The average size of QC-Mp nanoparticles was measured to be 66.05 nm and the average strand diameter and pore size of the 3D-printed construct were measured as 147.22 ± 5.83 and 388.44 ± 14.50 μm, respectively. The hemolysis rate of all scaffolds was below 2 %, indicating that they can be classified as non-hemolytic materials with sufficient blood compatibility. The PAH-Pc/QC-Mp scaffold exhibited significant antibacterial activity, enhanced cell viability in HaCat cells, sustained Mp release until day 7 (⁓60 %), and in-vivo wound healing promotion by stimulation of human keratinocytes. In conclusion, the proposed biocompatible construct demonstrates significant potential for the treatment of chronic and infected wounds by preventing infection and promoting accelerated wound healing.
尽管伤口敷料的研发取得了重大进展，但有效的皮肤修复仍然是一项重大挑战，这主要是由于反复感染这一持续存在的问题。整合了生物活性和抗菌剂的三维打印结构在应对这一挑战方面具有巨大潜力。在本研究中，制备了一种由聚烯丙胺盐酸盐（PAH）和果胶（Pc）组成，并掺入了载有莫匹罗星（Mp）的季铵化壳聚糖纳米颗粒（QC NPs）的三维打印水凝胶支架。本研究的主要目的是通过QC NPs实现Mp的可控和持续释放。测得QC-Mp纳米颗粒的平均尺寸为66.05 nm，三维打印结构的平均股直径和孔径分别为147.22±5.83和388.44±14.50μm。所有支架的溶血率均低于2%，表明它们可归类为具有足够血液相容性的非溶血材料。PAH-Pc/QC-Mp支架表现出显著的抗菌活性，提高了HaCat细胞的活力，Mp持续释放至第7天（约60%），并通过刺激人角质形成细胞促进体内伤口愈合。总之，所提出的生物相容性结构在预防感染和促进伤口加速愈合方面显示出治疗慢性和感染伤口的巨大潜力。
