Institute of Inflammation and Neurodegeneration, Medical Faculty, Otto-Von-Guericke University, Magdeburg, Germany.
Cellular Proteome Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Sci Rep. 2024 Oct 3;14(1):23032. doi: 10.1038/s41598-024-74228-4.
Extracellular vesicles (EVs) are key in intercellular communication, carrying biomolecules like nucleic acids, lipids, and proteins. This study investigated postprandial characteristics and proteomic profiles of blood-derived EVs in healthy individuals. Twelve participants fasted overnight before baseline assessments. After consuming a controlled isocaloric meal, EVs were isolated for proteomic and flow cytometric analysis. Plasma triacylglyceride levels confirmed fasting completion, while protein concentrations in plasma and EVs were monitored for postprandial stability. Proteomic analysis identified upregulated proteins related to transport mechanisms and epithelial/endothelial functions postprandially, indicating potential roles in physiological responses to nutritional intake. Enrichment analyses revealed vesicle-related pathways and immune system processes. Flow cytometry showed increased expression of CD324 on CD9CD63CD81 large extracellular vesicles postprandially, suggesting an epithelial origin. These findings offer valuable insights into postprandial EV dynamics and their potential physiological significance, highlighting the need for stringent fasting guidelines in EV studies to account for postprandial effects on EV composition and function.
细胞外囊泡 (EVs) 在细胞间通讯中起着关键作用，携带生物分子，如核酸、脂质和蛋白质。本研究调查了健康个体餐后血液衍生 EV 的特征和蛋白质组学特征。12 名参与者在基线评估前进行了一夜禁食。在摄入控制热量的餐后，分离 EVs 进行蛋白质组学和流式细胞术分析。血浆三酰甘油水平证实了禁食的完成，而血浆和 EVs 中的蛋白质浓度则监测餐后的稳定性。蛋白质组学分析发现了与运输机制和上皮/内皮功能相关的上调蛋白，提示其在对营养摄入的生理反应中具有潜在作用。富集分析显示了与囊泡相关的途径和免疫系统过程。流式细胞术显示，餐后 CD9+CD63+CD81 大细胞外囊泡上 CD324 的表达增加，提示其上皮来源。这些发现为餐后 EV 动力学及其潜在的生理意义提供了有价值的见解，强调在 EV 研究中需要严格的禁食指南，以考虑到餐后对 EV 组成和功能的影响。
