一种用于组胺和二胺氧化酶（DAO）活性的改进分光光度测定法。
An improved spectrophotometric assay for histamine and diamine oxidase (DAO) activity.
作者信息
Stoner P
出版信息
Agents Actions. 1985 Oct;17(1):5-9. doi: 10.1007/BF01966672.
Diamine oxidase or its substrates can be measured using a coupled reaction with peroxidase and the chromogen 3-methyl-2-benzothiazolone hydrazone (MBTH) with an appropriate acceptor such as 3-(dimethylamino)benzoic acid (DMAB). This method provides a more rapid and sensitive method of measuring histamine than current colorimetric assays; using this system, histamine was quantitated at concentrations of 10 to 400 mumol/l. The Km of DAO was determined to be 2.9 X 10(-5) M, a lower value than previously reported.
可以通过与过氧化物酶和显色剂3-甲基-2-苯并噻唑啉酮腙（MBTH）以及合适的受体（如3-（二甲基氨基）苯甲酸（DMAB））的偶联反应来测量二胺氧化酶或其底物。与目前的比色测定法相比，该方法提供了一种更快速、灵敏的组胺测量方法；使用该系统，组胺在10至400μmol/l的浓度下进行定量。DAO的Km值测定为2.9×10⁻⁵M，该值低于先前报道的值。
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